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Journal Article
Regulation of Dietary Fatty Acid Entrapment in Subcutaneous Adipose Tissue and Skeletal Muscle
2002
Overview
Regulation of Dietary Fatty Acid Entrapment in Subcutaneous Adipose Tissue and Skeletal Muscle
Kevin Evans 1 ,
Graham C. Burdge 2 ,
Stephen A. Wootton 2 ,
Mo L. Clark 3 and
Keith N. Frayn 3
1 Department of Clinical Chemistry, Staffordshire General Hospital, Stafford, U.K.
2 Institute of Human Nutrition, University of Southampton, Southampton, U.K.
3 Oxford Centre for Diabetes, Endocrinology, and Metabolism, Oxford, U.K.
Abstract
Using stable isotopic labeling of dietary fatty acids in conjunction with arteriovenous difference measurements, we have assessed
the regulation of lipoprotein lipase-derived fatty acid entrapment in subcutaneous adipose tissue and forearm muscle in healthy
subjects in the postprandial state. Eight volunteers fasted overnight and were then given a mixed meal containing [ 1- 13 C]palmitic acid and [1- 13 C]oleic acid. At baseline and for 6 h after the meal, blood samples were obtained from an arterialized hand vein and veins
draining subcutaneous abdominal adipose tissue and forearm muscle, and arteriovenous differences were calculated. Entrapment
of labeled fatty acids released by circulating triacylglycerol hydrolysis was close to 100% at 60 min, decreasing to 10–30%
by 360 min. Entrapment of labeled fatty acids in forearm muscle was >100% and did not change with time. This study shows that
entrapment of dietary fatty acids in adipose tissue in the postprandial period is a highly regulated process (varying with
time) and that this can be studied in humans using stable isotope- labeled fatty acids in combination with measurement of
appropriate arteriovenous differences. Also, fatty acid trapping in skeletal muscle is fundamentally different from that in
adipose tissue, in that all the fatty acids released by lipoprotein lipase in skeletal muscle are taken up by the tissue.
Footnotes
Address correspondence and reprint requests to Dr. Kevin Evans, Department of Clinical Chemistry, Staffordshire General Hospital,
Weston Road, Stafford, ST16 3SA UK. E-mail: kevin.evans{at}msgh-tr.wmids.nhs.uk .
Received for publication 7 January 2002 and accepted in revised form 4 June 2002.
ATBF, adipose tissue blood flow; A-V, arteriovenous difference; FAME, fatty acid methyl ester; GC-IRMS, gas chromatography-isotope
ratio mass spectrometry; HSL, hormone-sensitive lipase; LPL, lipoprotein lipase; NEFA, nonesterified fatty acid; TAG, triacylglycerol;
V-A, veno-arterial difference.
DIABETES
Publisher
American Diabetes Association
Subject
/ Adult
/ Biological and medical sciences
/ Body fat
/ Diabetes
/ Diet
/ Fatty Acids - administration & dosage
/ Fatty Acids, Nonesterified - blood
/ Fundamental and applied biological sciences. Psychology
/ Humans
/ Muscle, Skeletal - metabolism
/ Obesity
/ Plasma
/ Skin
/ Vertebrates: anatomy and physiology, studies on body, several organs or systems
