Journal Article

Cohesin and condensin extrude DNA loops in a cell cycle-dependent manner

Brugués, Jan, Quail, Thomas, Kimura, Hiroshi, Golfier, Stefan

2020

Overview

Loop extrusion by structural maintenance of chromosomes (SMC) complexes has been proposed as a mechanism to organize chromatin in interphase and metaphase. However, the requirements for chromatin organization in these cell cycle phases are different, and it is unknown whether loop extrusion dynamics and the complexes that extrude DNA also differ. Here, we used Xenopus egg extracts to reconstitute and image loop extrusion of single DNA molecules during the cell cycle. We show that loops form in both metaphase and interphase, but with distinct dynamic properties. Condensin extrudes DNA loops non-symmetrically in metaphase, whereas cohesin extrudes loops symmetrically in interphase. Our data show that loop extrusion is a general mechanism underlying DNA organization, with dynamic and structural properties that are biochemically regulated during the cell cycle.

• Publisher: eLife Science Publications, Ltd,eLife Sciences Publications Ltd,eLife Sciences Publications, Ltd
• Subject: Adenosine Triphosphatases - metabolism / Animals / Cell Biology / Cell Cycle / Cell Cycle Proteins - metabolism / Chromatin / Chromosomal Proteins, Non-Histone - metabolism / Chromosomes / Chromosomes and Gene Expression / Cohesin / Cohesins / Condensin / Deoxyribonucleic acid / DNA / DNA - chemistry / DNA - metabolism / DNA-Binding Proteins - metabolism / Gene expression / Interphase / loop extrusion / Metaphase / Multiprotein Complexes - metabolism / Nucleic Acid Conformation / single molecule / SMC / Xenopus laevis / Xenopus Proteins - metabolism