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Transcriptome characterization and expression profile of Coix lacryma-jobi L. in response to drought
by
Guo, Jihua
, Bao, Yingying
, Qin, Yan
, Zhang, Qingxia
, Miao, Guidong
in
Agricultural production
/ Analysis
/ Annotations
/ Biology
/ Biology and Life Sciences
/ Cereals
/ Coix
/ Coix lacryma-jobi
/ Computer and Information Sciences
/ Drought
/ Droughts
/ Engineering and Technology
/ Gene expression
/ Gene sequencing
/ Genes
/ Genetic aspects
/ Genetic diversity
/ Genetic transcription
/ Genomics
/ Influence
/ Ontology
/ Polyadenylation
/ Polymorphism
/ Research and analysis methods
/ Ribonucleic acid
/ RNA
/ Seeds
/ Transcription
/ Transcriptomes
2021
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Transcriptome characterization and expression profile of Coix lacryma-jobi L. in response to drought
by
Guo, Jihua
, Bao, Yingying
, Qin, Yan
, Zhang, Qingxia
, Miao, Guidong
in
Agricultural production
/ Analysis
/ Annotations
/ Biology
/ Biology and Life Sciences
/ Cereals
/ Coix
/ Coix lacryma-jobi
/ Computer and Information Sciences
/ Drought
/ Droughts
/ Engineering and Technology
/ Gene expression
/ Gene sequencing
/ Genes
/ Genetic aspects
/ Genetic diversity
/ Genetic transcription
/ Genomics
/ Influence
/ Ontology
/ Polyadenylation
/ Polymorphism
/ Research and analysis methods
/ Ribonucleic acid
/ RNA
/ Seeds
/ Transcription
/ Transcriptomes
2021
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Transcriptome characterization and expression profile of Coix lacryma-jobi L. in response to drought
by
Guo, Jihua
, Bao, Yingying
, Qin, Yan
, Zhang, Qingxia
, Miao, Guidong
in
Agricultural production
/ Analysis
/ Annotations
/ Biology
/ Biology and Life Sciences
/ Cereals
/ Coix
/ Coix lacryma-jobi
/ Computer and Information Sciences
/ Drought
/ Droughts
/ Engineering and Technology
/ Gene expression
/ Gene sequencing
/ Genes
/ Genetic aspects
/ Genetic diversity
/ Genetic transcription
/ Genomics
/ Influence
/ Ontology
/ Polyadenylation
/ Polymorphism
/ Research and analysis methods
/ Ribonucleic acid
/ RNA
/ Seeds
/ Transcription
/ Transcriptomes
2021
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Transcriptome characterization and expression profile of Coix lacryma-jobi L. in response to drought
Journal Article
Transcriptome characterization and expression profile of Coix lacryma-jobi L. in response to drought
2021
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Overview
Coix lacryma-jobi L. is a very important economic crop widely cultivated in Southeast Asia. Drought affects more than four million square kilometers every year, and is a significant factor limiting agricultural productivity. However, relatively little is known about how Coix lacryma-jobi L. responds to drought treatments. To obtain a detailed and comprehensive understanding of the mechanisms regulating the transcriptional responses of Coix lacryma-jobi L. to drought treatment, we employed high throughput short-read sequencing of cDNA prepared from polyadenylated RNA to explore global gene expression after a seven-day drought treatment. We generated a de novo assembled transcriptome comprising 65,480 unique sequences. Differential expression analysis based on RSEM-estimated transcript abundances identified 5,315 differentially expressed genes (DEGs) when comparing samples from plants following drought-treatment and from the appropriate controls. Among these, the transcripts for 3,460 genes were increased in abundance, whereas 1,855 were decreased. Real-time quantitative PCR for 5 transcripts confirmed the changes identified by RNA-Seq. The results provide a transcriptional overview of the changes in Coix lacryma-jobi L. in response to drought, and will be very useful for studying the function of associated genes and selection of molecular marker of Coix lacryma-jobi L in the future.
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