Journal Article

Genome-Wide identification and expression profiles of the WRKY transcription factor family in Artocarpus nanchuanensis

Zhang, Xiao, Chen, Yunli, Zhang, Huan, Li, Wenqiao, You, Shiqi, Zuo, Youwei, Xia, Changying, Zhang, Zhe, Deng, Hongping

2025

Overview

Background As one of the largest families of transcription factors (TFs), the WRKY gene family plays a significant role in plant growth, development, and response to hormone and environmental stress. Currently, there is no systematic analysis of the WRKY gene family in Artocarpus nanchuanensis . Results To explore its evolutionary mechanism and potential function, we performed a comprehensive analysis. A total of 113 AnWRKYs were identified, and 109 were mapped onto 28 chromosomes. These genes could be classified into 3 groups (Group I, Group IIa-IIe, Group III) based on phylogenetic analysis and the sequence characteristics of the AnWRKY domain. Syntenic analyses revealed 4 pairs of tandem repeats and 123 pairs of large fragment repeats. Furthermore, the expression profiles of AnWRKYs in roots, stems, and leaves showed that AnWRKYs had different spatial expression patterns. AnWRKY2 , AnWRKY14 , AnWRKY46 , AnWRKY55 , and AnWRKY105 may play important roles in the response to abiotic stress. In addition, subcellular localization analysis indicated that AnWRKY55 was localized in the nucleus. Conclusions This study is the first to report the identification and characterization of AnWRKYs in A. nanchuanensis and provides an important basis for future comprehensive evolutionary and functional analyses of AnWRKYs in A. nanchuanensis .

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Abiotic stress / Agriculture / Amino acids / Artocarpus / Artocarpus - genetics / Artocarpus - metabolism / Artocarpus nanchuanensis / Biomedical and Life Sciences / Botanical research / Breadfruits / Chromosomes / Cold / domain / Drought / Environmental stress / Expression profile / family / Gene expression / Gene Expression Profiling / Gene Expression Regulation, Plant / Genes / Genetic aspects / Genome, Plant / Genomes / Identification and classification / Life Sciences / Localization / Multigene Family / Phylogenetics / Phylogeny / Plant growth / Plant Proteins - genetics / Plant Proteins - metabolism / Plant Sciences / Proteins / Seeds / Software / Synteny / Transcription factors / Transcription Factors - genetics / Transcription Factors - metabolism / Tree Biology / WRKY gene family