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African swine fever virus MGF360-11L negatively regulates cGAS-STING-mediated inhibition of type I interferon production
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African swine fever virus MGF360-11L negatively regulates cGAS-STING-mediated inhibition of type I interferon production
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African swine fever virus MGF360-11L negatively regulates cGAS-STING-mediated inhibition of type I interferon production
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Journal Article
African swine fever virus MGF360-11L negatively regulates cGAS-STING-mediated inhibition of type I interferon production
2022
Overview
The type I interferon (IFN-I) signaling pathway is an important part of the innate immune response and plays a vital role in controlling and eliminating pathogens. African swine fever virus (ASFV) encodes various proteins to evade the host’s natural immunity. However, the molecular mechanism by which the ASFV-encoded proteins inhibit interferon production remains poorly understood. In the present study, ASFV MGF360-11L inhibited cGAS, STING, TBK1, IKKε, IRF7 and IRF3-5D mediated activation of the IFN-β and ISRE promoters, accompanied by decreases in IFN-β, ISG15 and ISG56 mRNA expression. ASFV MGF360-11L interacted with TBK1 and IRF7, degrading TBK1 and IRF7 through the cysteine, ubiquitin–proteasome and autophagy pathways. Moreover, ASFV MGF360-11L also inhibited the phosphorylation of TBK1 and IRF3 stimulated by cGAS-STING overexpression. Truncation mutation analysis revealed that aa 167-353 of ASFV MGF360-11L could inhibit cGAS-STING-mediated activation of the IFN-β and ISRE promoters. Finally, the results indicated that ASFV MGF360-11L plays a significant role in inhibiting IL-1β, IL-6 and IFN-β production in PAM cells (PAMs) infected with ASFV. In short, these results demonstrated that ASFV MGF360-11L was involved in regulating IFN-I expression by negatively regulating the cGAS signaling pathway. In summary, this study preliminarily clarified the molecular mechanism by which the ASFV MGF360-11L protein antagonizes IFN-I-mediated antiviral activity, which will help to provide new strategies for the treatment and prevention of ASF.
Publisher
BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
Subject
/ African Swine Fever - pathology
/ African Swine Fever - virology
/ African Swine Fever Virus - metabolism
/ Analysis
/ Animals
/ Biological response modifiers
/ Cloning
/ Cysteine
/ Hogs
/ IFN-I
/ Interferon Type I - genetics
/ Kinases
/ Medicine
/ Nucleotidyltransferases - genetics
/ Nucleotidyltransferases - metabolism
/ Plasmids
/ Proteins
/ Reagents
/ RNA
/ Swine
/ Vaccines
/ Veterinary Medicine/Veterinary Science
/ Virology
/ Viruses
