Asset Details
Do you wish to reserve the book?
Single-cell characterization of CRISPR-modified transcript isoforms with nanopore sequencing
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Single-cell characterization of CRISPR-modified transcript isoforms with nanopore sequencing
Do you wish to request the book?
Single-cell characterization of CRISPR-modified transcript isoforms with nanopore sequencing
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Single-cell characterization of CRISPR-modified transcript isoforms with nanopore sequencing
2021
Overview
We developed a single-cell approach to detect CRISPR-modified mRNA transcript structures. This method assesses how genetic variants at splicing sites and splicing factors contribute to alternative mRNA isoforms. We determine how alternative splicing is regulated by editing target exon-intron segments or splicing factors by CRISPR-Cas9 and their consequences on transcriptome profile. Our method combines long-read sequencing to characterize the transcript structure and short-read sequencing to match the single-cell gene expression profiles and gRNA sequence and therefore provides targeted genomic edits and transcript isoform structure detection at single-cell resolution.
Publisher
BioMed Central,Springer Nature B.V,BMC
Subject
/ Animal Genetics and Genomics
/ Biomedical and Life Sciences
/ Clustered Regularly Interspaced Short Palindromic Repeats
/ CRISPR
/ Exons
/ genome
/ Genomes
/ Genomics
/ gRNA
/ Humans
/ Isoforms
/ Kinases
/ Method
/ Microbial Genetics and Genomics
/ Nanopore Sequencing - methods
/ Protein Isoforms - metabolism
/ Receptors for Activated C Kinase
/ RNA, Guide, CRISPR-Cas Systems - metabolism
/ Single-cell long-read CRISPR screen
