Journal Article

Filter paper-based spin column method for cost-efficient DNA or RNA purification

Shi, Rui, Panthee, Dilip R., Lewis, Ramsey S.

2018

Overview

We describe herein a method of recharging used commercial spin columns or assembling homemade spin columns using filter paper as binding material for cost-effective, low throughput nucleic acid purification. The efficiency of filter paper-based spin columns was evaluated for purification of nucleic acids from various sources. Following protocols of commercial kits, we found filter paper to be a useful binding material for purification of nucleic acids, including plant genomic DNA, plant total RNA, PCR products, and DNA from agarose gels. However, filter paper has a weak binding affinity to plasmid DNA in tested miniprep protocols. Protocols for the use of filter paper recharged spin columns or homemade spin columns for low throughput purification of plant genomic DNA and total RNA with unused commercial kit buffers or less expensive homemade buffers are presented.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Analysis / Backup software / Binding / Biology and Life Sciences / Buffers / Cellulose / Centrifugation - methods / Crops / Deoxyribonucleic acid / DNA / DNA, Plant - chemistry / DNA, Plant - isolation & purification / Economic aspects / Engineering and Technology / Filter paper / Gels / Horticulture / Laboratories / Methods / Molecular biology / Nicotiana - chemistry / Nucleic acids / Physical Sciences / Plasmids / Purification / Recharging / Research and analysis methods / Ribonucleic acid / RNA / RNA, Plant - chemistry / RNA, Plant - isolation & purification / Soil sciences / Solanum lycopersicum - chemistry / Tobacco