Journal Article

Oxygen and mechanical ventilation impede the functional properties of resident lung mesenchymal stromal cells

Macias, Rolando, Vasquez, Margarita M., Mustafa, Shamimunisa B., Johnson-Pais, Teresa L., Gelfond, Jonathon A. L., Seidner, Steven R., Moreira, Alvaro G., Wilson, Desiree, Siddiqui, Sartaj K.

2020

Overview

Resident/endogenous mesenchymal stromal cells function to promote the normal development, growth, and repair of tissues. Following premature birth, the effects of routine neonatal care (e.g. oxygen support and mechanical ventilation) on the biological properties of lung endogenous mesenchymal stromal cells is (L-MSCs) is poorly understood. New Zealand white preterm rabbits were randomized into the following groups: (i) sacrificed at birth (Fetal), (ii) spontaneously breathing with 50% O2 for 4 hours (SB), or (iii) mechanical ventilation with 50% O2 for 4h (MV). At time of necropsy, L-MSCs were isolated, characterized, and compared. L-MSCs isolated from the MV group had decreased differentiation capacity, ability to form stem cell colonies, and expressed less vascular endothelial growth factor mRNA. Compared to Fetal L-MSCs, 98 and 458 genes were differentially expressed in the L-MSCs derived from the SB and MV groups, respectively. Gene ontology analysis revealed these genes were involved in key regulatory processes including cell cycle, cell division, and angiogenesis. Furthermore, the L-MSCs from the SB and MV groups had smaller mitochondria, nuclear changes, and distended endoplasmic reticula. Short-term hyperoxia/mechanical ventilation after birth alters the biological properties of L-MSCs and stimulates genomic changes that may impact their reparative potential.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Angiogenesis / Animals / Biological properties / Biology and Life Sciences / Birth / Cell cycle / Cell Differentiation - drug effects / Cell division / Cell Movement - drug effects / Cell Proliferation - drug effects / Comparative analysis / Endothelial growth factors / Endothelium / Female / Fetuses / Genes / Growth factors / Hyperoxia / Hyperoxia - metabolism / Instrument industry (Equipment) / International economic relations / Laboratory animals / Lung - metabolism / Lungs / Male / Mechanical ventilation / Medical equipment industry / Medicine and Health Sciences / Mesenchymal stem cells / Mesenchymal Stem Cells - metabolism / Mesenchymal Stem Cells - physiology / Mesenchyme / Messenger RNA / Mitochondria / mRNA / Necropsy / Neonates / Newborn infants / Ostomy / Oxygen / Oxygen - metabolism / Oxygen - physiology / Pediatrics / Physical Sciences / Premature birth / Premature infants / Rabbits / Research and Analysis Methods / Respiration, Artificial - adverse effects / Respiration, Artificial - methods / RNA / Stem cells / Stromal cells / Surfactants / Time / Vascular endothelial growth factor / Ventilation / Ventilators