Journal Article

Association between ustekinumab therapy and changes in specific anti-microbial response, serum biomarkers, and microbiota composition in patients with IBD: A pilot study

Hercogova, Jana Tresnak, Schierova, Dagmar, Jackova, Zuzana, Rob, Filip, Tlaskalova-Hogenova, Helena, Drastich, Pavel, Lukas, Milan, Roubalova, Radka, Thon, Tomas, Stehlikova, Zuzana, Mihula, Martin, Kolar, Martin, Coufal, Stepan, Vasatko, Martin, Kreisinger, Jakub, Novakova, Michaela, Jiraskova Zakostelska, Zuzana, Kverka, Miloslav, Bajer, Lukas, Kostovcikova, Klara

2022

Overview

Ustekinumab, is a new therapy for patients with IBD, especially for patients suffering from Crohn's disease (CD) who did not respond to anti-TNF treatment. To shed light on the longitudinal effect of ustekinumab on the immune system, we investigated the effect on skin and gut microbiota composition, specific immune response to commensals, and various serum biomarkers. We recruited 11 patients with IBD who were monitored over 40 weeks of ustekinumab therapy and 39 healthy controls (HC). We found differences in the concentrations of serum levels of osteoprotegerin, TGF-β1, IL-33, and serum IgM antibodies against Lactobacillus plantarum between patients with IBD and HC. The levels of these biomarkers did not change in response to ustekinumab treatment or with disease improvement during the 40 weeks of observation. Additionally, we identified differences in stool abundance of uncultured Subdoligranulum, Faecalibacterium, and Bacteroides between patients with IBD and HC. In this preliminary study, we provide a unique overview of the longitudinal monitoring of fecal and skin microbial profiles as well as various serum biomarkers and humoral and cellular response to gut commensals in a small cohort of patients with IBD on ustekinumab therapy.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Analysis / Antibodies / Antigens / Antiinfectives and antibacterials / Bacteria / Biological markers / Biological products / Biology and Life Sciences / Biomarkers / Care and treatment / Commensals / Composition / Computer and Information Sciences / Crohn Disease - therapy / Crohn's disease / Crohns disease / Cytokines / Disease / Ethylenediaminetetraacetic acid / Feces / Humans / Immune response / Immune system / Immunoglobulin M / Immunoglobulins / Immunotherapy / Inflammatory bowel disease / Inflammatory bowel diseases / Intestinal microflora / Medical research / Medicine and Health Sciences / Medicine, Experimental / Microbiota / Microbiota (Symbiotic organisms) / Microorganisms / Monoclonal antibodies / Osteoprotegerin / Patient outcomes / Pilot Projects / Remission (Medicine) / Serum levels / Skin / Therapy / TNF inhibitors / Transforming growth factor-b1 / Transforming growth factors / Tumor necrosis factor / Tumor Necrosis Factor Inhibitors / Tumor necrosis factor-TNF / Ustekinumab - therapeutic use