Journal Article

Dynamic proteomic profiling of human periodontal ligament stem cells during osteogenic differentiation

Wang, Zhifa, Wu, Buling, Chen, Zhao, Li, Jianjia, Liu, Wei, Wang, Runting, Wang, Zhaodan, Fang, Fuchun, Qiu, Wei, Chen, Zehao, Huang, Xiangyu

2021

Overview

Background Human periodontal ligament stem cells (hPDLSCs) are ideal seed cells for periodontal regeneration. A greater understanding of the dynamic protein profiles during osteogenic differentiation contributed to the improvement of periodontal regeneration tissue engineering. Methods Tandem Mass Tag quantitative proteomics was utilized to reveal the temporal protein expression pattern during osteogenic differentiation of hPDLSCs on days 0, 3, 7 and 14. Differentially expressed proteins (DEPs) were clustered and functional annotated by Gene Ontology (GO) terms. Pathway enrichment analysis was performed based on the Kyoto Encyclopedia of Genes and Genomes database, followed by the predicted activation using Ingenuity Pathway Analysis software. Interaction networks of redox-sensitive signalling pathways and oxidative phosphorylation (OXPHOS) were conducted and the hub protein SOD2 was validated with western blotting. Results A total of 1024 DEPs were identified and clustered in 5 distinctive clusters representing dynamic tendencies. The GO enrichment results indicated that proteins with different tendencies show different functions. Pathway enrichment analysis found that OXPHOS was significantly involved, which further predicted continuous activation. Redox-sensitive signalling pathways with dynamic activation status showed associations with OXPHOS to various degrees, especially the sirtuin signalling pathway. SOD2, an important component of the sirtuin pathway, displays a persistent increase during osteogenesis. Data are available via ProteomeXchange with identifier PXD020908. Conclusion This is the first in-depth dynamic proteomic analysis of osteogenic differentiation of hPDLSCs. It demonstrated a dynamic regulatory mechanism of hPDLSC osteogenesis and might provide a new perspective for research on periodontal regeneration. Graphical abstract

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Analysis / Biomedical and Life Sciences / Biomedical Engineering and Bioengineering / Cell Biology / Cell differentiation / Dynamic proteomics / Experiments / Gene expression / Genes / Genomes / Genomics / Human periodontal ligament stem cell / Life Sciences / Ligaments / Ontology / Osteogenesis / Osteogenic differentiation / Oxidative phosphorylation / Periodontal ligament / Periodontium / Phosphorylation / Protein expression / Proteins / Proteomics / Regenerative Medicine/Tissue Engineering / Signal transduction / SOD2 / Stem cell research / Stem Cells / Superoxide dismutase / Tissue engineering / Western blotting