Journal Article

Brassinosteroids is involved in methane-induced adventitious root formation via inducing cell wall relaxation in marigold

Li, Yihua, Hua, Jun, Wang, Chunlei, Huang, Dengjing, Zhang, Hongsheng, Yao, Yandong, Qi, Nana, Liao, Weibiao, Hou, Xuemei, Li, Changxia

2023

Overview

Background Methane (CH 4 ) and brassinosteroids (BRs) are important signaling molecules involved in a variety of biological processes in plants. Results Here, marigold ( Tagetes erecta L. ‘Marvel’) was used to investigate the role and relationship between CH 4 and BRs during adventitious root (AR) formation. The results showed a dose-dependent effect of CH 4 and BRs on rooting, with the greatest biological effects of methane-rich water (MRW, CH 4 donor) and 2,4-epibrassinolide (EBL) at 20% and 1 μmol L − 1 , respectively. The positive effect of MRW on AR formation was blocked by brassinoazole (Brz, a synthetic inhibitor of EBL), indicating that BRs might be involved in MRW-regulated AR formation. MRW promoted EBL accumulation during rooting by up-regulating the content of campestanol (CN), cathasterone (CT), and castasterone (CS) and the activity of Steroid 5α-reductase (DET2), 22α-hydroxylase (DWF4), and BR-6-oxidase (BR6ox), indicating that CH 4 could induce endogenous brassinolide (BR) production during rooting. Further results showed that MRW and EBL significantly down-regulated the content of cellulose, hemicellulose and lignin during rooting and significantly up-regulated the hydrolase activity, i.e. cmcase, xylanase and laccase. In addition, MRW and EBL also significantly promoted the activity of two major cell wall relaxing factors, xyloglucan endotransglucosylase/hydrolase (XTH) and peroxidase, which in turn promoted AR formation. While, Brz inhibited the role of MRW on these substances. Conclusions BR might be involved in CH 4 -promoted AR formation by increasing cell wall relaxation.

• Publisher: BioMed Central,BioMed Central Ltd,BMC
• Subject: 2,4-epibrassinolide / Adventitious root formation / adventitious roots / Agriculture / Biomedical and Life Sciences / brassinolide / Brassinosteroids - pharmacology / Cell wall relaxation / cell walls / Cellulose / Chemical properties / dose response / Growth / hemicellulose / Hydrolases / Influence / laccase / Life Sciences / Ligands (Biochemistry) / lignin / Marigolds / methane / Methane - pharmacology / Methane-rich water / peroxidase / Plant Roots - physiology / Plant Sciences / Roots (Botany) / Tagetes erecta / Tree Biology / xylanases / xyloglucan:xyloglucosyl transferase