Journal Article

Genome-wide identification and characterization of GATA gene family involved in ovule development of Korean pine (Pinus koraiensis)

Zhang, Yue, Yang, Ling, Zhao, Caihong

2025

Overview

Background GATA transcription factors, a type of zinc-finger DNA-binding protein, play a crucial role in regulating various biological processes including plant growth, stress response, and hormone signaling by controlling the expression of target genes. Recently, GATA genes have been discovered in numerous plant species. However, they have not yet been reported in Korean pine ( Pinus koraiensis ). Results In this study, a total of 15 PkorGATA genes were identified in the whole genome of Korean pine. These PkorGATA genes are unevenly distributed across nine chromosomes and are categorized into four subfamilies based on their conserved structural domains. The results of cis-acting element analysis, RNA-seq, and qRT-PCR indicate that PkorGATA genes play a significant role in the developmental processes of Korean pine ovules. Moreover, subcellular localization results revealed that PkorGATA2 , PkorGATA3 , PkorGATA8 , PkorGATA9 , PkorGATA10 and PkorGATA11 are localized in the nucleus. The transcriptional activation activity of six PkorGATA proteins in yeast showed that PkorGATA2, PkorGATA3, and PkorGATA10 exhibit transcriptional activation capabilities, suggesting that their potential function as transcription factors within the nucleus. Conclusion In general, this study provides candidate gene resources for functional exploration of GATA genes.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Abscisic acid / Agriculture / Amino acid sequence / Analysis / Biological activity / Biomedical and Life Sciences / Biosynthesis / Cellular stress response / Chlorophyll / Chromosomes / Diseases and pests / DNA binding proteins / DNA-binding protein / Drought / Expression analysis / family / GATA gene / GATA gene family / GATA Transcription Factors - genetics / GATA Transcription Factors - metabolism / Gene expression / Gene Expression Regulation, Plant / Genes / Genes, Plant / Genetic aspects / Genetic resources / Genome, Plant / Genomes / Growth / Korean pine (Pinus koraiensis) / Life Sciences / Metabolism / Methods / Multigene Family / Nitrogen / Nuclei (cytology) / Ovule - genetics / Ovule - growth & development / Ovule development / Ovules / Phylogenetics / Phylogeny / Pine / Pinus - genetics / Pinus - growth & development / Pinus koraiensis / Plant growth / Plant Proteins - genetics / Plant Proteins - metabolism / Plant Sciences / Plant species / Proteins / regulatory sequences / Seeds / sequence analysis / Signal transduction / Sorghum / species / stress response / Transcription activation / Transcription factors / transcriptional activation / Tree Biology / yeasts / Zinc finger proteins