Journal Article

The Reality of Pervasive Transcription

Dinger, Marcel E., Morillon, Antonin, Gerstein, Mark B., Schlesinger, Felix J., Ponting, Chris P., Amaral, Paulo P., Carninci, Piero, Hayashizaki, Yoshihide, Mattick, John S., Wahlestedt, Claes, Rinn, John L., Stadler, Peter F., Rozowsky, Joel S., Gingeras, Thomas R., Clark, Michael B., Taft, Ryan J., Morris, Kevin V.

2011

Overview

In parallel, whole chromosome tiling array interrogation of the RNA content of a variety of human tissues and cell lines revealed that, collectively, at least 93% of genomic bases are transcribed in one cell type or another [1],[10]-[13]. Since it is well established that highly expressed mRNAs dominate the non-ribosomal portion of the polyA+ transcriptome [7],[8],[10],[14]-[19], normalization approaches were used to reduce the quantity of highly expressed transcripts in these cDNA analyses [7],[8], and are implicit in tiling array approaches. [...]any estimate of the pervasiveness of transcription requires inclusion of all data sources, and less than exhaustive analyses can only provide lower bounds for transcriptional complexity.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Animals / Arrays / Databases, Genetic / DNA sequencing / DNA, Complementary - analysis / DNA, Complementary - chemistry / DNA, Complementary - genetics / Epigenomics / Expressed Sequence Tags - chemistry / Genetic transcription / Genetics and Genomics/Bioinformatics / Genetics and Genomics/Gene Discovery / Genetics and Genomics/Genomics / Genome / Genomes / Humans / Life Sciences / Mammals / Medical research / Nucleotide sequencing / Oligonucleotide Array Sequence Analysis - methods / Physiological aspects / Proteins / RNA / Sequence Analysis, RNA - methods / Transcription, Genetic