Journal Article

High-resolution detection of chromosomal rearrangements in leukemias through mate pair whole genome sequencing

Nilsson, Daniel, Ivanov Öfverholm, Ingegerd, Lötstedt, Britta, Tran, Anh Nhi, Zachariadis, Vasilios, Vezzi, Francesco, Nordgren, Ann, Taylan, Fulya, Lindstrand, Anna, Nordenskjöld, Magnus, Barbany, Gisela

2018

Overview

The detection of recurrent somatic chromosomal rearrangements is standard of care for most leukemia types. Even though karyotype analysis-a low-resolution genome-wide chromosome analysis-is still the gold standard, it often needs to be complemented with other methods to increase resolution. To evaluate the feasibility and applicability of mate pair whole genome sequencing (MP-WGS) to detect structural chromosomal rearrangements in the diagnostic setting, we sequenced ten bone marrow samples from leukemia patients with recurrent rearrangements. Samples were selected based on cytogenetic and FISH results at leukemia diagnosis to include common rearrangements of prognostic relevance. Using MP-WGS and in-house bioinformatic analysis all sought rearrangements were successfully detected. In addition, unexpected complexity or additional, previously undetected rearrangements was unraveled in three samples. Finally, the MP-WGS analysis pinpointed the location of chromosome junctions at high resolution and we were able to identify the exact exons involved in the resulting fusion genes in all samples and the specific junction at the nucleotide level in half of the samples. The results show that our approach combines the screening character from karyotype analysis with the specificity and resolution of cytogenetic and molecular methods. As a result of the straightforward analysis and high-resolution detection of clinically relevant rearrangements, we conclude that MP-WGS is a feasible method for routine leukemia diagnostics of structural chromosomal rearrangements.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Analysis / Biochemistry / Bioinformatics / Biology and Life Sciences / Biophysics / Bone Marrow / Cancer genetics / Chromosome Aberrations / Chromosome abnormalities / Chromosome rearrangements / Computational Biology / Development and progression / Diagnostic systems / DNA sequencing / Early Detection of Cancer / Exons / Feasibility Studies / Gene sequencing / Genetic aspects / Genetic research / Genetics / Genomes / Genomics / High resolution / Hospitals / Humans / Identification and classification / In Situ Hybridization, Fluorescence / Karyotypes / Laboratories / Leukemia / Leukemia - genetics / Leukemia - pathology / Medical diagnosis / Medicine / Medicine and Health Sciences / Political aspects / Research and Analysis Methods / Surgery / Whole Genome Sequencing - methods