Journal Article

GhPYL9-5D and GhPYR1-3 A positively regulate Arabidopsis and cotton responses to ABA, drought, high salinity and osmotic stress

Ma, Caixia, Chen, Pengyun, Hao, Fushun, Wang, Yibin, Li, Yunxiang, Zhang, Gaofeng, Sun, Lirong, Yin, Shanshan, Zhou, Huimin

2023

Overview

Background Abscisic acid (ABA) receptor pyrabactin resistance 1/PYR1-like/regulatory components of ABA receptor proteins (PYR/PYL/RCARs) have been demonstrated to play pivotal roles in ABA signaling and in response to diverse environmental stimuli including drought, salinity and osmotic stress in Arabidopsis . However, whether and how GhPYL9-5D and GhPYR1-3A, the homologues of Arabidopsis PYL9 and PYR1 in cotton, function in responding to ABA and abiotic stresses are still unclear. Results GhPYL9-5D and GhPYR1-3A were targeted to the cytoplasm and nucleus. Overexpression of GhPYL9-5D and GhPYR1-3A in Arabidopsis wild type and sextuple mutant pyr1pyl1pyl2pyl4pyl5pyl8 plants resulted in ABA hypersensitivity in terms of seed germination, root growth and stomatal closure, as well as seedling tolerance to water deficit, salt and osmotic stress. Moreover, the VIGS (Virus-induced gene silencing) cotton plants, in which GhPYL9-5D or GhPYR1-3A were knocked down, showed clearly reduced tolerance to polyethylene glycol 6000 (PEG)-induced drought, salinity and osmotic stresses compared with the controls. Additionally, transcriptomic data revealed that GhPYL9-5D was highly expressed in the root, and GhPYR1-3A was strongly expressed in the fiber and stem. GhPYL9-5D , GhPYR1-3A and their homologs in cotton were highly expressed after treatment with PEG or NaCl, and the two genes were co-expressed with redox signaling components, transcription factors and auxin signal components. These results suggest that GhPYL9-5D and GhPYR1-3A may serve important roles through interplaying with hormone and other signaling components in cotton adaptation to salt or osmotic stress. Conclusions GhPYL9-5D and GhPYR1-3A positively regulate ABA-mediated seed germination, primary root growth and stomatal closure, as well as tolerance to drought, salt and osmotic stresses likely through affecting the expression of multiple downstream stress-associated genes in Arabidopsis and cotton.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: ABA receptor protein / ABA response / Abiotic stress / Abscisic acid / Abscisic Acid - metabolism / Abscisic Acid - pharmacology / Acid resistance / Agriculture / Analysis / Arabidopsis / Arabidopsis - metabolism / auxins / Biomedical and Life Sciences / Carrier Proteins - genetics / Composition / Cotton / Cytoplasm / DNA binding proteins / Drought / Droughts / Environmental aspects / Environmental effects / Flowers & plants / Gene Expression Regulation, Plant / Gene silencing / Genes / Genetic aspects / Genetic engineering / Germination / Germination - genetics / Gossypium - genetics / Gossypium - metabolism / Homology / Hypersensitivity / Kinases / Life Sciences / Methods / mutants / Osmosis / Osmotic Pressure / Osmotic stress / Plant growth / Plant Sciences / Plants, Genetically Modified - genetics / Polyethylene glycol / Proteins / Receptors / root growth / Salinity / Salinity effects / Salt stress (Botany) / Salts / Seed germination / Seedlings / Seeds / Senescence / Signal transduction / Signaling / Sodium chloride / Sodium Chloride - metabolism / Stomata / Stomatal closure / stomatal movement / Stress, Physiological - genetics / Stresses / Transcription factors / Transcriptomics / Transgenic plants / Tree Biology / Water deficit