Journal Article

Comparative genomic analysis of the multispecies probiotic-marketed product VSL#3

Mora, Diego, Wels, Michiel, de Vos, Willem M., Douillard, François P., Eijlander, Robyn T.

2018

Overview

Several probiotic-marketed formulations available for the consumers contain live lactic acid bacteria and/or bifidobacteria. The multispecies product commercialized as VSL#3 has been used for treating various gastro-intestinal disorders. However, like many other products, the bacterial strains present in VSL#3 have only been characterized to a limited extent and their efficacy as well as their predicted mode of action remain unclear, preventing further applications or comparative studies. In this work, the genomes of all eight bacterial strains present in VSL#3 were sequenced and characterized, to advance insights into the possible mode of action of this product and also to serve as a basis for future work and trials. Phylogenetic and genomic data analysis allowed us to identify the 7 species present in the VSL#3 product as specified by the manufacturer. The 8 strains present belong to the species Streptococcus thermophilus, Lactobacillus acidophilus, Lactobacillus paracasei, Lactobacillus plantarum, Lactobacillus helveticus, Bifidobacterium breve and B. animalis subsp. lactis (two distinct strains). Comparative genomics revealed that the draft genomes of the S. thermophilus and L. helveticus strains were predicted to encode most of the defence systems such as restriction modification and CRISPR-Cas systems. Genes associated with a variety of potential probiotic functions were also identified. Thus, in the three Bifidobacterium spp., gene clusters were predicted to encode tight adherence pili, known to promote bacteria-host interaction and intestinal barrier integrity, and to impact host cell development. Various repertoires of putative signalling proteins were predicted to be encoded by the genomes of the Lactobacillus spp., i.e. surface layer proteins, LPXTG-containing proteins, or sortase-dependent pili that may interact with the intestinal mucosa and dendritic cells. Taken altogether, the individual genomic characterization of the strains present in the VSL#3 product confirmed the product specifications, determined its coding capacity as well as identified potential probiotic functions.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Bacteria / Bacterial Adhesion / Bifidobacterium - classification / Bifidobacterium - genetics / Biology and Life Sciences / Care and treatment / Commercialization / Comparative analysis / Comparative studies / CRISPR / CRISPR-Cas Systems / Data analysis / Data processing / Dendritic cells / Disease / Ecology and Environmental Sciences / Formulations / Gastrointestinal diseases / Gene clusters / Genetic aspects / Genome, Bacterial / Genomes / Genomic analysis / Genomics / Gram-positive bacteria / Humans / Inflammatory bowel disease / Intestinal Mucosa - microbiology / Intestine / Laboratories / Laboratorium voor Microbiologie / Lactic acid / Lactic acid bacteria / Lactobacilli / Lactobacillus / Lactobacillus - classification / Lactobacillus - genetics / Lactobacillus acidophilus / Lactobacillus acidophilus - classification / Lactobacillus acidophilus - genetics / Lactobacillus helveticus / Lactobacillus paracasei / Lactobacillus paracasei - classification / Lactobacillus paracasei - genetics / Lactobacillus plantarum / Lactobacillus plantarum - classification / Lactobacillus plantarum - genetics / Lactobacillus rhamnosus / Medicine and Health Sciences / Microbial Sensitivity Tests / Microbiological Laboratory / Microbiologie / Microbiology / Mode of action / Mucosa / Phylogeny / Physiological aspects / Pili / Probiotics / Probiotics - classification / Product specifications / Proteins / Research and Analysis Methods / Signaling / Sortase / Strains (organisms) / Streptococcus salivarius / Surface boundary layer / Trends / VLAG / WIMEK / Yogurt