Journal Article

Purification and Characterization of Plantaricin ZJ5, a New Bacteriocin Produced by Lactobacillus plantarum ZJ5

Gu, Qing, Zhu, Mu-Yuan, Song, Da-Feng

2014

Overview

The aim of this study is to investigate the antimicrobial potential of Lactobacillus plantarum ZJ5, a strain isolated from fermented mustard with a broad range of inhibitory activity against both Gram-positive and Gram-negative bacteria. Here we present the peptide plantaricin ZJ5 (PZJ5), which is an extreme pH and heat-stable. However, it can be digested by pepsin and proteinase K. This peptide has strong activity against Staphylococcus aureus. PZJ5 has been purified using a multi-step process, including ammonium sulfate precipitation, cation-exchange chromatography, hydrophobic interactions and reverse-phase chromatography. The molecular mass of the peptide was found to be 2572.9 Da using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The primary structure of this peptide was determined using amino acid sequencing and DNA sequencing, and these analyses revealed that the DNA sequence translated as a 44-residue precursor containing a 22-amino-acid N-terminal extension that was of the double-glycine type. The bacteriocin sequence exhibited no homology with known bacteriocins when compared with those available in the database, indicating that it was a new class IId bacteriocin. PZJ5 from a food-borne strain may be useful as a promising probiotic candidate.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Acids / Amino Acid Sequence / Amino acids / Ammonium / Ammonium salts / Ammonium sulfate / Antibiosis / Antimicrobial agents / Bacteria / Bacteriocins / Bacteriocins - biosynthesis / Bacteriocins - chemistry / Bacteriocins - genetics / Bacteriocins - isolation & purification / Base Sequence / Biochemistry / Biology and Life Sciences / Brassicaceae / Cation exchanging / Cation-exchange chromatography / Chemical properties / Chromatography / Deoxyribonucleic acid / DNA / DNA sequencing / Endopeptidase K / Enterococcus faecalis / Fermented food / Food / Gene sequencing / Glycine / Gram-negative bacteria / Gram-positive bacteria / Heat exchange / Homology / Hydrophobicity / Identification / Ionization / Laboratories / Lactobacillus plantarum / Lactobacillus plantarum - genetics / Lactobacillus plantarum - metabolism / Life sciences / Mass spectrometry / Mass spectroscopy / Molecular Sequence Data / Molecular Weight / Mustard / Nucleotide sequence / Pepsin / Peptides / Physiology / Precipitation (Meteorology) / Probiotics / Proteinase / Ribosomal DNA / Sulfates / Thermal stability