Journal Article

Characterization of biliary microbiota dysbiosis in extrahepatic cholangiocarcinoma

Sobhani, Iradj, Rodriguez, Christophe, Khonsari, Mahmood Reza, Sohrabi, Masoudreza, Saab, Massa, Mestivier, Denis, Faraji, Amirhossein

2021

Overview

Extrahepatic cholangiocarcinoma (CCA) accounts for 3% of digestive cancers. The role of biliary microbiota as an environment-related modulator has been scarcely investigated in CCA, and the putative impact of associated diseases has not been yet assessed. We characterized the biliary microbiota in CCA patients in order to identify a specific CCA-related dysbiosis. The biliary effluents were collected through an endoscopic retrograde pancreatic cholangiography (ERCP) examination involving 28 CCA and 47 patients with gallstones, herein considered as controls. The biliary effluents were submitted to bacterial DNA extraction and 16S rRNA sequencing, using Illumina technology. Overall, 32% of CCA and 22% of controls displayed another associated disease, such as diabetes, pancreatitis, inflammatory bowel disease, or primary sclerosing cholangitis. Such associated diseases were considered in the comparisons that were made. Principal coordinate analysis (PCoA) detected a significant disparity of biliary microbiota composition between CCA patients and controls without an associated disease. Amongst the most abundant phyla, Proteobacteria did not significantly differ between CCA patients and controls, whereas Firmicutes levels were lower and Bacteroidetes higher in CCAs’ biliary microbiota than in the controls’ microbiota. The most abundant genera were Enterococcus , Streptococcus , Bacteroides , Klebsiella , and Pyramidobacte r in CCA’s biliary microbiota. Additionally, levels of Bacteroides , Geobacillus , Meiothermus , and Anoxybacillus genera were significantly higher in CCA patients’ biliary microbiota, without an associated disease, in comparison with controls. A specific CCA-related dysbiosis was identified as compared to controls independently from associated diseases. This suggests that a microorganism community may be involved in CCA pathogenesis.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Bile / Bile ducts / Biology and Life Sciences / Campylobacter / Cellular biology / Cholangiocarcinoma / Colon / Colon cancer / Colorectal cancer / Deoxyribonucleic acid / DNA / Dysbacteriosis / Dysbiosis / Editing / Electronic mail / Endoscopy / Environmental factors / Expressed sequence tags / Gastroenterology / Gastrointestinal cancer / Gene sequencing / Genetic aspects / Health aspects / Hepatitis / Hospitals / Liver / Liver diseases / Markers / Medical prognosis / Medical research / Medicine and Health Sciences / Metagenomics / Microbiota / Microbiota (Symbiotic organisms) / Microorganisms / Pathology / Research facilities / Ribonucleic acid / RNA / rRNA / Tumors / Visualization