Journal Article

Irisin Promotes Human Umbilical Vein Endothelial Cell Proliferation through the ERK Signaling Pathway and Partly Suppresses High Glucose-Induced Apoptosis

Wang, Xing Li, Song, Haibo, Cui, Taixing, Wang, Fang, Tang, Dongqi, Wang, Zhongde, Zhang, Yuzhu, Jiang, Miao, Zhang, Mingxiang, Yang, Lijun, Wu, Fei, Zhang, Yuan, Li, Shiwu

2014

Overview

Irisin is a newly discovered myokine that links exercise with metabolic homeostasis. It is involved in modest weight loss and improves glucose intolerance. However, the direct effects and mechanisms of irisin on vascular endothelial cells (ECs) are not fully understood. In the current study, we demonstrated that irisin promoted Human Umbilical Vein Endothelial Cell (HUVEC) proliferation. It was further demonstrated that this pro-proliferation effect was mediated by irisin-induced activation of extracellular signal-related kinase (ERK) signaling pathways. Inhibition of ERK signaling with U0126 decreased the pro-proliferation effect of irisin on HUVECs. It was also demonstrated that irisin reduced high glucose-induced apoptosis by up-regulating Bcl-2 expression and down-regulating Bax, Caspase-9 and Caspase-3 expression. In summary, these results suggested that irisin plays a novel role in sustaining endothelial homeostasis by promoting HUVEC proliferation via the ERK signaling pathway and protects the cell from high glucose-induced apoptosis by regulating Bcl-2,Bax and Caspase expression.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Apoptosis / Apoptosis - drug effects / Bcl-2 protein / bcl-2-Associated X Protein - genetics / bcl-2-Associated X Protein - metabolism / Biology / Biology and Life Sciences / Breast cancer / Butadienes - pharmacology / Caspase / Caspase 3 - genetics / Caspase 3 - metabolism / Caspase 9 - genetics / Caspase 9 - metabolism / Caspase-3 / Caspase-9 / Cell cycle / Cell growth / Cell proliferation / Cell Proliferation - drug effects / Childrens health / Diabetes / Disease / Endocrinology / Endothelial cells / Endothelium / Extracellular signal-regulated kinase / Extracellular Signal-Regulated MAP Kinases - antagonists & inhibitors / Extracellular Signal-Regulated MAP Kinases - genetics / Extracellular Signal-Regulated MAP Kinases - metabolism / Fibronectins - genetics / Fibronectins - metabolism / Fibronectins - pharmacology / Gene Expression Regulation / Glucose / Glucose - antagonists & inhibitors / Glucose - metabolism / Glucose - pharmacology / Glucose tolerance / Homeostasis / Hospitals / Human Umbilical Vein Endothelial Cells - cytology / Human Umbilical Vein Endothelial Cells - drug effects / Human Umbilical Vein Endothelial Cells - metabolism / Humans / Intolerance / Kinases / Laboratories / Maternal & child health / Medicine / Medicine and Health Sciences / Metabolic disorders / Nitriles - pharmacology / Protein Kinase Inhibitors - pharmacology / Proteins / Proto-Oncogene Proteins c-bcl-2 - genetics / Proto-Oncogene Proteins c-bcl-2 - metabolism / Recombinant Proteins - genetics / Recombinant Proteins - metabolism / Recombinant Proteins - pharmacology / Reproductive health / Signal Transduction / Signaling / Stem cells / Studies / Umbilical vein / Veins & arteries