Journal Article

Transcriptome characterization of candidate genes for heat tolerance in perennial ryegrass after exogenous methyl Jasmonate application

He, Jie, Wang, Yang, Zhou, Jie, Huang, Linkai, Nie, Gang, Liao, Zongchao, Feng, Guangyan, Wang, Xia, Jiang, Yiwei, Appiah, Charlotte, Li, Dandan, Zhang, Xinquan

2022

Overview

Methyl jasmonate (MeJA) plays a role in improving plant stress tolerance. The molecular mechanisms associated with heat tolerance mediated by MeJA are not fully understood in perennial grass species. The study was designed to explore transcriptomic mechanisms underlying heat tolerance by exogenous MeJA in perennial ryegrass ( Lolium perenne L.) using RNA-seq. Transcriptomic profiling was performed on plants under normal temperature (CK), high temperature for 12 h (H), MeJA pretreatment (T), MeJA pretreatment + H (T-H), respectively. The analysis of differentially expressed genes (DEGs) showed that H resulted in the most DEGs and T had the least, compared with CK. Among them, the DEGs related to the response to oxygen-containing compound was higher in CKvsH, while many genes related to photosynthetic system were down-regulated. The DEGs related to plastid components was higher in CKvsT. GO and KEGG analysis showed that exogenous application of MeJA enriched photosynthesis related pathways under heat stress. Exogenous MeJA significantly increased the expression of genes involved in chlorophyll (Chl) biosynthesis and antioxidant metabolism, and decreased the expression of Chl degradation genes, as well as the expression of heat shock transcription factor - heat shock protein (HSF-HSP) network under heat stress. The results indicated that exogenous application of MeJA improved the heat tolerance of perennial ryegrass by mediating expression of genes in different pathways, such as Chl biosynthesis and degradation, antioxidant enzyme system, HSF-HSP network and JAs biosynthesis.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Acclimatization (Plants) / Acetates - metabolism / Acetates - pharmacology / Agriculture / Analysis / antioxidant enzymes / Antioxidants / Antioxidants - metabolism / Biomedical and Life Sciences / Biosynthesis / Chlorophyll / Chlorophyll - genetics / Chlorophyll - metabolism / Cyclopentanes - metabolism / Cyclopentanes - pharmacology / Degradation / Diseases and pests / Environmental aspects / Gene expression / Gene Expression Profiling / gene expression regulation / Gene Expression Regulation, Plant - drug effects / Gene Ontology / Gene Regulatory Networks / Genes / Genetic transcription / Genomes / Grasses / Growth / Heat resistance / Heat shock factors / Heat shock proteins / Heat stress / Heat tolerance / High temperature / Homeostasis / HSF-HSP network / Life Sciences / Lolium - drug effects / Lolium - genetics / Lolium perenne / Lolium perenne L / MeJA / Methods / Methyl jasmonate / Molecular mechanics / Molecular modelling / Observations / Ontology / Oxylipins - metabolism / Oxylipins - pharmacology / perennial grasses / Photosynthesis / Physiology / Plant growth / Plant Growth Regulators - pharmacology / Plant heat tolerance / Plant Proteins - genetics / Plant Sciences / Plant stress / Pretreatment / Reproducibility of Results / Ryegrasses / Seeds / Senescence / sequence analysis / species / stress tolerance / temperature / Thermotolerance - drug effects / Thermotolerance - genetics / transcription factors / transcriptome / Transcriptomes / Transcriptomics / Tree Biology