Journal Article

Transcriptome and metabolome analysis reveals mechanism of light intensity modulating iridoid biosynthesis in Gentiana macrophylla Pall

Wang, Yaomin, Wang, Li, Yue, Zhenggang, Yang, Ye, Wang, Fang, Fu, Huanhuan, Mi, Fakai, He, Yihan

2024

Overview

Light intensity is a key factor affecting the synthesis of secondary metabolites in plants. However, the response mechanisms of metabolites and genes in Gentiana macrophylla under different light intensities have not been determined. In the present study, G. macrophylla seedlings were treated with LED light intensities of 15 µmol/m 2 /s (low light, LL), 90 µmol/m 2 /s (medium light, ML), and 200 µmol/m 2 /s (high light, HL), and leaves were collected on the 5th day for further investigation. A total of 2162 metabolites were detected, in which, the most abundant metabolites were identified as flavonoids, carbohydrates, terpenoids and amino acids. A total of 3313 and 613 differentially expressed genes (DEGs) were identified in the LL and HL groups compared with the ML group, respectively, mainly enriched in KEGG pathways such as carotenoid biosynthesis, carbon metabolism, glycolysis/gluconeogenesis, amino acids biosynthesis, plant MAPK pathway and plant hormone signaling. Besides, the transcription factors of GmMYB5 and GmbHLH20 were determined to be significantly correlated with loganic acid biosynthesis; the expression of photosystem-related enzyme genes was altered under different light intensities, regulating the expression of enzyme genes involved in the carotenoid, chlorophyll, glycolysis and amino acids pathway, then affecting their metabolic biosynthesis. As a result, low light inhibited photosynthesis, delayed glycolysis, thus, increased certain amino acids and decreased loganic acid production, while high light got an opposite trend. Our research contributed significantly to understand the molecular mechanism of light intensity in controlling metabolic accumulation in G. macrophylla .

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Acid production / Acids / Agriculture / Amino acids / Biomedical and Life Sciences / Biosynthesis / Carbohydrates / carbon metabolism / Carotenoids / Chlorophyll / Correlation analysis / DNA binding proteins / Environmental aspects / enzymes / Flavonoids / Gene expression / Gene Expression Profiling / gene expression regulation / Gene Expression Regulation, Plant / Genes / Genetic aspects / Genomes / Genomics / Gentian root / Gentiana / Gentiana - genetics / Gentiana - metabolism / Gentiana macrophylla / Gentiana macrophylla Pall / Gluconeogenesis / Glycolysis / Herbal medicine / Identification and classification / International economic relations / Iridoid biosynthesis / Iridoids / Iridoids - metabolism / Isoflavones / Leaves / Life Sciences / Light / Light intensity / Light-emitting diodes / Luminous intensity / MAP kinase / Metabolism / Metabolites / Metabolome - radiation effects / Metabolome analysis / metabolomics / Molecular modelling / Photosynthesis / Photosystem / Physiological aspects / Plant genetics / Plant hormones / Plant Leaves - genetics / Plant Leaves - metabolism / Plant Leaves - radiation effects / Plant metabolites / Plant Sciences / Scientific equipment and supplies industry / Secondary metabolites / Seedlings / Terpenes / Transcription factors / Transcriptome / Transcriptome analysis / Transcriptomes / Tree Biology