Journal Article

Identification of NHXs in Gossypium species and the positive role of GhNHX1 in salt tolerance

Zhao, Jing-Ruo, Ma, Xiao-Nan, Xu, Fu-Chun, Gao, Wei, Long, Lu, Guo, Dan-Dan, Yang, Wen-Wen

2020

Overview

Background Plant Na + /H + antiporters (NHXs) are membrane-localized proteins that maintain cellular Na + /K + and pH homeostasis. Considerable evidence highlighted the critical roles of NHX family in plant development and salt response; however, NHXs in cotton are rarely studied. Results The comprehensive and systematic comparative study of NHXs in three Gossypium species was performed. We identified 12, 12, and 23 putative NHX proteins from G. arboreum , G. raimondii , and G. hirsutum , respectively. Phylogenetic study revealed that repeated polyploidization of Gossypium spp. contributed to the expansion of NHX family. Gene structure analysis showed that cotton NHXs contain many introns, which will lead to alternative splicing and help plants to adapt to high salt concentrations in soil. The expression changes of NHX s indicate the possible differences in the roles of distinct NHX s in salt response. GhNHX1 was proved to be located in the vacuolar system and intensively induced by salt stress in cotton. Silencing of GhNHX1 resulted in enhanced sensitivity of cotton seedlings to high salt concentrations, which suggests that GhNHX1 positively regulates cotton tolerance to salt stress. Conclusion We characterized the gene structure, phylogenetic relationship, chromosomal location, and expression pattern of NHX genes from G. arboreum , G. raimondii , and G. hirsutum . Our findings indicated that the cotton NHX genes are regulated meticulously and differently at the transcription level with possible alternative splicing. The tolerance of plants to salt stress may rely on the expression level of a particular NHX , rather than the number of NHXs in the genome. This study could provide significant insights into the function of plant NHX s, as well as propose promising candidate genes for breeding salt-resistant cotton cultivars.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Abiotic stress / Agriculture / Alternative splicing / Amino Acid Sequence / antiporters / Biomedical and Life Sciences / Comparative analysis / Comparative studies / comparative study / Cotton / Crop breeding / Crops / Cultivars / Flowers & plants / Gene expression / Genes / Genetically altered foods / Genomes / Genomics / Gossypium / Gossypium - genetics / Gossypium - metabolism / Gossypium arboreum / Gossypium hirsutum / Gossypium raimondii / Homeostasis / Introns / Irrigation / Kinases / Life Sciences / Membrane protein / Membrane proteins / Phylogenetics / Phylogeny / Plant breeding / plant development / Plant growth / Plant Proteins - genetics / Plant Proteins - metabolism / Plant Sciences / Plant-abiotic interactions / Polyploidy / potassium / Proteins / Research Article / Rice / Salinity tolerance / Salt / salt stress / Salt Tolerance / Seedlings / Sensitivity enhancement / sodium / sodium-hydrogen antiporter / Sodium-Hydrogen Exchangers - genetics / Sodium-Hydrogen Exchangers - metabolism / soil / Soil salinity / Soil salinization / Splicing / Structural analysis / Studies / Transcription / Transcription (Genetics) / Transporters / Tree Biology / vacuoles