Journal Article

Radical Change in Zoonotic Abilities of Atypical BSE Prion Strains as Evidenced by Crossing of Sheep Species Barrier in Transgenic Mice

Marín-Moreno, Alba, Aguilar-Calvo, Patricia, Huor, Alvina, Interactions hôtes-agents pathogènes [Toulouse] (IHAP) ; Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT) ; Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT), Tillier, Cecile, Food Standards AgencyFS231051Spanish GovernmentRTA2012-00004AGL2016-78054-RBES-2010-040922Fundacie La Marati5 de TV3201821-31Instituto Nacional de Investigacian y Tecnologia Agraria y AgroalimeritariaINIA-FP1-SGIT-2015-02Alliance BioSecure Research FoundationFAI3S FRM-2014FEDER programs POCTEFAEFA148/16, Cassard, Hervé, Interactions hôtes-agents pathogènes [Toulouse] (IHAP) ; Ecole Nationale Vétérinaire de Toulouse (ENVT) ; Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT)-In

2020

Overview

Classical bovine spongiform encephalopathy (BSE) is the only zoonotic prion disease described to date. Although the zoonotic potential of atypical BSE prions have been partially studied, an extensive analysis is still needed. We conducted a systematic study by inoculating atypical BSE isolates from different countries in Europe into transgenic mice overexpressing human prion protein (PrP): TgMet(129), TgMet/Val(129), and TgVal(129). L-type BSE showed a higher zoonotic potential in TgMet(129) mice than classical BSE. whereas Val(1)(29)-PrP variant was a strong molecular protector against L-type BSE prions, even in heterozygosis. H-type BSE could not be transmitted to any of the mice. We also adapted 1 H- and 1 L-type BSE isolate to sheep-PrP transgenic mice and inoculated them into human-PrP transgenic mice. Atypical BSE prions showed a modification in their zoonotic ability after adaptation to sheep-PrP producing agents able to infect TgMet(129) and TgVal(129) bearing features that make them indistinguishable of sporadic Creutzfeldt-Jakob disease prions.

• Publisher: Centers for Disease Control and Prevention,CCSD,U.S. National Center for Infectious Diseases
• Subject: Adaptation / Analysis / Animal genetic engineering / atypical bovine spongiform encephalopathy / Bovine spongiform encephalopathy / Brain research / BSE / Cattle / Creutzfeldt-Jakob disease / Disease / Ethics / Experiments / Food chains / Glucose / Life Sciences / prion / Prions / Prions (Proteins) / Proteins / Radical Change in Zoonotic Abilities of Atypical BSE Prion Strains as Evidenced by Crossing of Sheep Species Barrier in Transgenic Mice / Rodents / Sheep / Transgenic animals / transmissible spongiform encephalopathies / transmission barrier / Val129-PrP / Virulence / Zoonoses
• ISBN: 0005415139000