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Whole-Genome Random Sequencing and Assembly of Haemophilus Influenzae Rd

by Phillips, Cheryl A. , Venter, J. Craig , Kerlavage, Anthony R. , McDonald, Lisa A. , Fritchman, Janice L. , Sutton, Granger , Gocyne, Jeannie D. , Utterback, Teresa R. , Kirkness, Ewen F. , Cotton, Matthew D. , Shirley, Robert , Hanna, Michael C. , Small, Keith V. , Scott, John , Weidman, Janice F. , Brandon, Rhonda C. , Bult, Carol J. , Geoghagen, N. S. M. , White, Owen , Dougherty, Brian A. , Clayton, Rebecca A. , Fields, Chris , Merrick, Joseph M. , Tomb, Jean-Francois , Kelley, Jenny M. , Fraser, Claire M. , Spriggs, Tracy , Glodek, Anna , Saudek, Deborah M. , Nguyen, David T. , Liu, Li-Ing , Fine, Leah D. , Hedblom, Eva , FitzHugh, Will , Fuhrmann, Joyce L. , Gnehm, Cheryl L. , Smith, Hamilton O. , Adams, Mark D. , McKenney, Keith , Fleischmann, Robert D.

in Amino acids / Bacteria / Bacterial Proteins - genetics / Bacteriology / Base Composition / Base Sequence / Biological and medical sciences / Chromosome mapping / Chromosome Mapping - methods / Chromosomes, Bacterial / Cloning, Molecular / Costs and Cost Analysis / Databases, Factual / DNA / DNA, Bacterial - genetics / Fundamental and applied biological sciences. Psychology / Genes, Bacterial / Genetic aspects / Genetics / Genome, Bacterial / Genomes / Haemophilus influenzae / Haemophilus influenzae - genetics / Haemophilus influenzae - physiology / Hemophilus influenzae / Libraries / Membrane transport proteins / Microbiology / Molecular Sequence Data / Nuclear membrane / Operator regions / Operon / Pilot Projects / Repetitive Sequences, Nucleic Acid / Research Article / RNA, Bacterial - genetics / RNA, Ribosomal - genetics / Sequence Analysis, DNA - methods / Sequencing / Software / Sperm transport / Terminator regions

1995

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Whole-Genome Random Sequencing and Assembly of Haemophilus Influenzae Rd

1995

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Whole-Genome Random Sequencing and Assembly of Haemophilus Influenzae Rd

1995

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Journal Article

Whole-Genome Random Sequencing and Assembly of Haemophilus Influenzae Rd

Phillips, Cheryl A.,

Venter, J. Craig,

Kerlavage, Anthony R.,

McDonald, Lisa A.,

Fritchman, Janice L.,

Sutton, Granger,

Gocyne, Jeannie D.,

Utterback, Teresa R.,

Kirkness, Ewen F.,

Cotton, Matthew D.,

Shirley, Robert,

Hanna, Michael C.,

Small, Keith V.,

Scott, John,

Weidman, Janice F.,

Brandon, Rhonda C.,

Bult, Carol J.,

Geoghagen, N. S. M.,

White, Owen,

Dougherty, Brian A.,

Clayton, Rebecca A.,

Fields, Chris,

Merrick, Joseph M.,

Tomb, Jean-Francois,

Kelley, Jenny M.,

Fraser, Claire M.,

Spriggs, Tracy,

Glodek, Anna,

Saudek, Deborah M.,

Nguyen, David T.,

Liu, Li-Ing,

Fine, Leah D.,

Hedblom, Eva,

FitzHugh, Will,

Fuhrmann, Joyce L.,

Gnehm, Cheryl L.,

Smith, Hamilton O.,

Adams, Mark D.,

McKenney, Keith,

Fleischmann, Robert D.

1995

Overview

An approach for genome analysis based on sequencing and assembly of unselected pieces of DNA from the whole chromosome has been applied to obtain the complete nucleotide sequence (1,830, 137 base pairs) of the genome from the bacterium Haemophilus influenzae Rd. This approach eliminates the need for initial mapping efforts and is therefore applicable to the vast array of microbial species for which genome maps are unavailable. The H. influenzae Rd genome sequence (Genome Sequence DataBase accession number L42023) represents the only complete genome sequence from a free-living organism.

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Publisher

American Society for the Advancement of Science,American Association for the Advancement of Science,The American Association for the Advancement of Science

Subject

Amino acids

/ Bacteria

/ Bacterial Proteins - genetics

/ Bacteriology

/ Base Composition

/ Base Sequence

/ Biological and medical sciences

/ Chromosome mapping

/ Chromosome Mapping - methods

/ Chromosomes, Bacterial

/ Cloning, Molecular