Asset Details
Do you wish to reserve the book?
Validation of reference genes for quantitative real-time PCR during leaf and flower development in Petunia hybrida
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Validation of reference genes for quantitative real-time PCR during leaf and flower development in Petunia hybrida
Do you wish to request the book?
Validation of reference genes for quantitative real-time PCR during leaf and flower development in Petunia hybrida
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Validation of reference genes for quantitative real-time PCR during leaf and flower development in Petunia hybrida
2010
Overview
Background: Identification of genes with invariant levels of gene expression is a prerequisite for validating transcriptomic changes accompanying development. Ideally expression of these genes should be independent of the morphogenetic process or environmental condition tested as well as the methods used for RNA purification and analysis. Results: In an effort to identify endogenous genes meeting these criteria nine reference genes (RG) were tested in two Petunia lines (Mitchell and V30). Growth conditions differed in Mitchell and V30, and different methods were used for RNA isolation and analysis. Four different software tools were employed to analyze the data. We merged the four outputs by means of a non-weighted unsupervised rank aggregation method. The genes identified as optimal for transcriptomic analysis of Mitchell and V30 were EF1α in Mitchell and CYP in V30, whereas the least suitable gene was GAPDH in both lines. Conclusions: The least adequate gene turned out to be GAPDH indicating that it should be rejected as reference gene in Petunia. The absence of correspondence of the best-suited genes suggests that assessing reference gene stability is needed when performing normalization of data from transcriptomic analysis of flower and leaf development.
Publisher
BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
Subject
/ Biomedical and Life Sciences
/ Flowers
/ Flowers - growth & development
/ Gene Expression Regulation, Developmental
/ Gene Expression Regulation, Plant
/ genetics
/ methods
/ Petunia
/ Petunia - growth & development
/ Plant Leaves - growth & development
/ Proteins
/ Reverse Transcriptase Polymerase Chain Reaction
/ Reverse Transcriptase Polymerase Chain Reaction - methods
/ Reverse Transcriptase Polymerase Chain Reaction - standards
/ Software
/ Studies
