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大熊猫源猫泛白细胞减少症病毒VP2基因的测序及遗传进化分析
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大熊猫源猫泛白细胞减少症病毒VP2基因的测序及遗传进化分析
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Journal Article
大熊猫源猫泛白细胞减少症病毒VP2基因的测序及遗传进化分析
2026
Overview
为探究大熊猫源猫泛白细胞减少症病毒（Feline panleukopenia virus， FPV）流行毒株的分子流行特征，本研究对一例腹泻大熊猫（Ailuropoda melanoleuca）粪便样本进行检测。通过PCR成功扩增并测定了FPV VP2基因全长序列（命名为Giant panda/AB/2025）。遗传进化分析显示，该毒株属于FPV G1基因型，与FPV参考毒株核苷酸同源性为99.2%～99.9%，与研究团队2018年分离的大熊猫源FPV株同源性可达99.4%。关键氨基酸位点分析发现，决定宿主特异性的位点（80、93、103、323、564和568）均与典型FPV一致，但其91位氨基酸由A突变为S（A91S）。蛋白3D结构模型预测显示，A91S突变导致VP2蛋白表面Loop区局部构象改变。结果表明，我国部分地区的大熊猫种群中已存在携带A91S突变的FPV变异株感染，需加强持续监测。本研究结果为大熊猫FPV的流行病学和疫苗研发提供了数据参考。
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