Journal Article

Circular RNA hsa_(c)irc₀000730 restrains cell proliferation, migration, and invasion in cervical cancer through miR‐942‐5p/PTEN axis

Wang, Jian, Yuan, Dan‐Dan, Yan, Ming‐Yu, Jia, Cun‐De

2021

Overview

Circular RNAs (circRNAs) play prominent roles in regulating the progression of cancers. This study is aimed to decipher the role of hsa_(c)irc₀000730 in cervical cancer (CC).The differentially expressed circRNAs of CC were screened out from the Gene Expression Omnibus database. qRT‐PCR was used to detect circ₀000730 expression in CC tissues and cell lines, and the Kaplan–Meier curve was adopted to figure out the relationship between circ₀00730 expression and the overall survival time of CC patients. BrdU assay and Tanswell assay were utilized to examine the proliferation, migration, and invasion of CC cells. Western blot was adopted to detect PTEN protein expression. Bioinformatics analysis and dual‐luciferase reporter assay were used to examine the target relationship between miR‐942‐5p and circ₀000730 or PTEN, respectively.Circ₀000730 was among the differentially expressed circRNAs in CC. Circ₀000730 was significantly down‐regulated in the cancer tissues of 50 CC patients and CC cell lines. Additionally, underexpression of circ₀000730 was associated with the shorter survival time of CC patients. Gain‐ and loss‐of‐function assays highlighted that circ₀000730 significantly inhibited the proliferation, migration, and invasion of CC cells. Mechanistically, miR‐942‐5p was identified as a downstream target of circ₀000730, and circ₀000730 could positively regulate PTEN expression via repressing miR‐942‐5p in CC cells.Circ₀000730 inhibits the proliferation, migration, and invasion of CC cells via regulating miR‐942‐5p/PTEN axis. Circ₀000730 probably acts as a tumor suppressor in CC, and it may be a candidate target for the treatment of CC.

• Publisher: Wiley Publishing Asia Pty Ltd,Wiley
• Subject: cervical cancer / Circ₀000730 / miR‐942‐5p / Original / proliferation / PTEN