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Diferenciacion de especie micobacteriana por FT-IR
by
Coronado Ríos, Sandra Milena
, Cuéllar Gil, Jorge Andrés
, Arrubla Qco, Roberto Carlos
in
BIOLOGY
/ E coli
2011
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Diferenciacion de especie micobacteriana por FT-IR
by
Coronado Ríos, Sandra Milena
, Cuéllar Gil, Jorge Andrés
, Arrubla Qco, Roberto Carlos
in
BIOLOGY
/ E coli
2011
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Journal Article
Diferenciacion de especie micobacteriana por FT-IR
2011
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Overview
Se trabajó con espectroscopía infrarroja transformada de Fourier (FT-IR) para diferenciar diez especies de micobacterias. Mycobacterium intracelullare y M. fortuitum (ATCC), M. flavensces, M. smegmatis, M. chelone, M. gordonae, M. triviale, M. vaccae, M. terrae y M. nonchromogenicum (IP). Como control de diferenciación de género se incluyó Staphylococcus aureus, Streptococcus viridans y Streptococcus pyogenes, Klebsiella pneumoniae y Escherichia coli, cada especie se corrió por triplicado en KBr y ATR. Los espectros se analizaron según el método de diferenciación de componentes principales, y se realizaron derivadas de primer orden (D1) en modalidad de transmisión usando la pastilla de KBr y la base ATR, además se diseñó una biblioteca espectral con la primera derivada de ATR. La sensibilidad de detección fue de 100% al trabajar con KBr y el nivel de diferenciación fue de 100% en tres de cuatro muestras problema.
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