Journal Article

The Impact of Different Cell Culture Mediums on CD8+ T Cells Expansion: A Bioinformatics Study

Bereimipour, Ahmad, Jalili, Arsalan, Hajifathali, Abbas, Roshandel, Elham, Aghdami, Nasser

2022

Overview

Different Cell Culture medias can affect the expansion of T cells. The aim of this study is to assess signaling pathways, protein interactions and genes in T cells cultured in different common T cell expansion medias to select the best candidate. In this in silico observational study, with the use of bioinformatics analysis and the use of enrichment databases, gene expression profiles were investigated using microarray analysis. The results of this study were the joint selection of 26 upregulated genes and 59 downregulated genes that were involved in SREBP control of lipid synthesis, co-stimulatory signal during T-cell activation mitosis and chromosome dynamics, telomeres, telomerase, and cellular aging signal pathways. Using bioinformatics analyzes, integrated and regular genes were selected as common genes and inhibitor, interleukin 7 and 15 expansion media.

• Publisher: Royan Institute of Iran,Royan Institute,Royan Institute (ACECR), Tehran
• Subject: AKT protein / Bioinformatics / CD8 antigen / CD80 antigen / Cell activation / Cell culture / Cell division / Chromosomes / Cytokines / Datasets / Developmental biology / DNA microarrays / expansion / Gene expression / Genes / Interleukin 7 / Kinases / Lipids / Lymphocytes / Lymphocytes T / Medical research / microarray / Mitosis / Motility / Observational studies / Ontology / Original / Protein interaction / Proteins / Regenerative medicine / Research centers / RNA polymerase / Science / Signal transduction / Stem cells / Sterol regulatory element-binding protein / T cell receptors / tcd8+ cells / Telomerase / Telomeres