Asset Details
Do you wish to reserve the book?
A new tandem repeat-based genotyping scheme for the global surveillance of Xanthomonas citri pv. mangiferaeindicae, an understudied bacterial pathogen of major importance to mango and cashew production
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
A new tandem repeat-based genotyping scheme for the global surveillance of Xanthomonas citri pv. mangiferaeindicae, an understudied bacterial pathogen of major importance to mango and cashew production
Do you wish to request the book?
A new tandem repeat-based genotyping scheme for the global surveillance of Xanthomonas citri pv. mangiferaeindicae, an understudied bacterial pathogen of major importance to mango and cashew production
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
A new tandem repeat-based genotyping scheme for the global surveillance of Xanthomonas citri pv. mangiferaeindicae, an understudied bacterial pathogen of major importance to mango and cashew production
2025
Overview
Bacterial canker, caused by Xanthomonas citri pv. mangiferaeindicae (Xcm), is a disease that has a devastating impact on mango and cashew industries in many regions. Yet, despite its agricultural importance for these Anacardiaceae species, Xcm has been neglected. Little is known about its epidemiology, evolution and molecular interactions with host plants. The most relevant studies reporting its genetic structure were primarily based on amplified fragment length polymorphism (AFLP) data. This technique provides reliable assessments of the genetic relatedness among bacteria, but is limited in terms of interlaboratory comparisons. Alternative genotyping techniques are required to decipher the global epidemiology and geographic expansion of Xcm. Herein, we screened the genome of the Xcm strain CFBP1716 for tandem repeats. We developed and evaluated the performance of an optimized Multi Locus Variable number of tandem repeat Analysis (MLVA), targeting 16 tandem repeat loci primarily with large repeat units, i.e., minisatellites (MLVA-16). To achieve this, we genotyped a comprehensive collection of 152 Xcm strains, representative of the pathogen's worldwide genetic diversity, together with some reference strains of X. citri pv. anacardii, another genetically-related pathogen of Anacardiaceae. MLVA-16 allowed us to distinguish the two pathovars. Although MLVA-16 was slightly less discriminative than AFLP, the two derived datasets were strongly correlated, suggesting that MLVA-16 provides a good phylogenetic signal. Five clusters with some geographic coherence were delineated, based on discriminant analysis of principal components. The two major clusters grouped strains from multiple geographic origins. In contrast, all strains that have emerged on mango or cashew in West Africa grouped in one cluster, which did not contain any strains of different origin. MLVA-16 represents an opportunity to improve our understanding of the structure of Xcm populations, by sharing genotyping data. The MLVA-16 data generated in this study was deposited in a dedicated online database.
Publisher
Public Library of Science,Public Library of Science (PLoS)
Subject
