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Saccharomyces cerevisiae ACR2 gene encodes an arsenate reductase
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Journal Article
Saccharomyces cerevisiae ACR2 gene encodes an arsenate reductase
1998
Overview
The
ACR2 gene of
Saccharomyces cerevisiae was disrupted by insertion of a
HIS3 gene. Cells with the disruption were sensitive to arsenate. This phenotype could be complemented by
ACR2 on a plasmid. The
ACR2 gene was cloned and expressed in
Escherichia coli as a
malE gene fusion with a C-terminal histidine tag. The combination of chimeric MBP-Acr2-6H protein and yeast cytosol from an
ACR2-disrupted strain exhibited arsenate reductase activity.
Publisher
Elsevier B.V
Subject
