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Molecular diversity of arbuscular mycorrhizal fungi colonising arable crops
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Molecular diversity of arbuscular mycorrhizal fungi colonising arable crops
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Journal Article
Molecular diversity of arbuscular mycorrhizal fungi colonising arable crops
2001
Overview
We used differences in small subunit ribosomal RNA genes to identify groups of arbuscular mycorrhizal fungi that are active in the colonisation of plant roots growing in arable fields around North Yorkshire, UK. Root samples were collected from four arable fields and four crop species, fungal sequences were amplified from individual plants by the polymerase chain reaction using primers NS31 and AM1. The products were cloned and 303 clones were classified by their restriction pattern with
HinfI or
RsaI; 72 were subsequently sequenced. Colonisation was dominated by
Glomus species with a preponderance of only two sequence types, which are closely related. There is evidence for seasonal variation in colonisation in terms of both level of colonisation and sequence types present. Fungal diversity was much lower than that previously reported for a nearby woodland.
Publisher
Elsevier B.V
