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The KlPGS1 gene encoding phosphatidylglycerolphosphate synthase in Kluyveromyces lactis is essential and assigned to chromosome I
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The KlPGS1 gene encoding phosphatidylglycerolphosphate synthase in Kluyveromyces lactis is essential and assigned to chromosome I
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Journal Article
The KlPGS1 gene encoding phosphatidylglycerolphosphate synthase in Kluyveromyces lactis is essential and assigned to chromosome I
2004
Overview
The phosphatidylglycerolphosphate synthase (CDP-diacylglycerol:
sn-glycerol-3-phosphate 3-phosphatidyltransferase, EC 2.7.8.5) is an essential enzyme in biosynthesis of cardiolipin. In this work we report the isolation, heterological cloning, molecular characterization and physical mapping of the
Saccharomyces cerevisiae PEL1/PGS1 homologue from
Kluyveromyces lactis. The
pel1 mutant strain of
S. cerevisiae was used to isolate this homologue by screening a
K. lactis genomic library. The novel cloned gene was named
KlPGS1. Its coding region was found to consist of 1623 bp. The corresponding protein exhibits 55% amino acid identity to its
S. cerevisiae counterpart. The presence of the mitochondrial presequence indicates its mitochondrial localization. Sporulation and ascus dissection of diploids heterozygous for single-copy disruption of
KlPGS1 revealed that the
KlPGS1 gene, is essential in
K. lactis. Using a DIG-dUTP-labeled DNA probe-originated from the
KlPGS1 gene and Southern hybridization of contour-clamped homogeneous electric field (CHEF)-separated
K. lactis chromosomal DNA, the
KlPGS1 gene was assigned to chromosome I. The nucleotide sequence data reported in this paper were submitted to GenBank and assigned the Accession No.
AY176328.
Publisher
Elsevier B.V,Blackwell Publishing Ltd,Oxford University Press
