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Heterologous production of a laccase from the basidiomycete Pycnoporus cinnabarinus in the dimorphic yeast Yarrowia lipolytica

by Asther, Marcel , Chamkha, Mohamed , Beckerich, Jean-Marie , Otterbein, Ludovic , Madzak, Catherine , Moukha, Serge , Gaillardin, Claude

in Aspergillus / Basidiomycetes / Basidiomycota - enzymology / Basidiomycota - genetics / Bioreactors / Cell culture / Culture Media / Cytology / Dimorphism / Genetic engineering / Genetic transformation / Glycosylation / Growth rate / Heterologous expression / Host systems / Industrial applications / Industrial Microbiology / LAC1 gene / Laccase / Laccase - genetics / Laccase - isolation & purification / Laccase - metabolism / Life Sciences / Microbiology and Parasitology / Pichia / Protein secretion / Pycnoporus cinnabarinus / Recombinant Proteins - genetics / Recombinant Proteins - isolation & purification / Recombinant Proteins - metabolism / Secretion signals / Yarrowia - enzymology / Yarrowia - genetics / Yarrowia lipolytica

2005

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Heterologous production of a laccase from the basidiomycete Pycnoporus cinnabarinus in the dimorphic yeast Yarrowia lipolytica

by Asther, Marcel , Chamkha, Mohamed , Beckerich, Jean-Marie , Otterbein, Ludovic , Madzak, Catherine , Moukha, Serge , Gaillardin, Claude

2005

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Heterologous production of a laccase from the basidiomycete Pycnoporus cinnabarinus in the dimorphic yeast Yarrowia lipolytica

by Asther, Marcel , Chamkha, Mohamed , Beckerich, Jean-Marie , Otterbein, Ludovic , Madzak, Catherine , Moukha, Serge , Gaillardin, Claude

2005

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Journal Article

Heterologous production of a laccase from the basidiomycete Pycnoporus cinnabarinus in the dimorphic yeast Yarrowia lipolytica

Asther, Marcel,

Chamkha, Mohamed,

Beckerich, Jean-Marie,

Otterbein, Ludovic,

Madzak, Catherine,

Moukha, Serge,

Gaillardin, Claude

2005

Overview

Pycnoporus cinnabarinus lac1 gene was expressed in Yarrowia lipolytica. Different secretion signals and culture media were tested. Production was correlated to both culture growth rate and cell morphology (highest at low growth rate, without mycelium). Recombinant laccase was characterized (immunodetection, N-terminal sequencing) and purified. Production was estimated to 20 mg l −1 in a bioreactor. Thus, complex metalloenzymes can be produced in Yarrowia, assuming some control of host physiology. Lac1p production was compared in Yarrowia, Pichia and Aspergillus: recombinant proteins were active, but host systems differed in transformation efficiency, production, and glycosylation. If not the best producer, Yarrowia offers very high transformation efficiencies, allowing the genetic engineering of laccases for industrial applications.

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Publisher

Elsevier B.V,Blackwell Publishing Ltd,Oxford University Press,Oxford University Press (OUP)

Subject

Aspergillus

/ Basidiomycetes

/ Basidiomycota - enzymology

/ Basidiomycota - genetics

/ Bioreactors

/ Cell culture

/ Culture Media