Journal Article

A new method to estimate photosynthetic parameters through net assimilation rate-intercellular space CO2 concentration (A-C-i) curve and chlorophyll fluorescence measurements

Moualeu-Ngangue, Dany P, Stuetzel, Hartmut, Institute for Horticultural Production Systems, Vegetable Systems Modelling Section ; Leibniz Universität Hannover = Leibniz University Hannover, Chen, Tsu Wei, Écophysiologie des Plantes sous Stress environnementaux (LEPSE) ; Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)

2017

Overview

Gas exchange (GE) and chlorophyll fluorescence (CF) measurements are widely used to noninvasively study photosynthetic parameters, for example the rates of maximum Rubisco carboxylation (V-cmax), electron transport rate (J), daytime respiration (R-d) and mesophyll conductance (g(m)). Existing methods for fitting GE data (net assimilation rate-intercellular space CO2 concentration (A-C-i) curve) are based on two assumptions: g(m) is unvaried with CO2 concentration in the intercellular space (C-i); and light absorption () and the proportion of quanta absorbed by photosystem II () are constant in the data set. These may result in significant bias in estimating photosynthetic parameters. To avoid the above-mentioned hypotheses, we present a new method for fitting A-C-i curves and CF data simultaneously. This method was applied to a data set obtained from cucumber (Cucumis sativus) leaves of various leaf ages and grown under eight different light conditions. The new method had significantly lower root mean square error and a lower rate of failures compared with previously published methods (6.72% versus 24.1%, respectively) and the effect of light conditions on V-cmax and J was better observed. Furthermore, the new method allows the estimation of a new parameter, the fraction of incoming irradiance harvested by photosystem II, and the dependence of g(m) on C-i.

• Publisher: Wiley,CCSD,New Phytologist Trust,Wiley Subscription Services, Inc
• Subject: absorption / A–Ci curves / Carbon dioxide / carboxylation / Chlorophyll / chlorophyll fluorescence / cucumbers / Cucumis sativus / data collection / electron transfer / extracellular space / fitting method / Fluorescence / Gas exchange / leaves / Life Sciences / light intensity / mesophyll / mesophyll conductance / Methods / photosynthetic parameters / photosystem II / ribulose-bisphosphate carboxylase / Vegetal Biology
• ISBN: 0003938754000