Journal Article

Influence of Some Heterocyclic, Cyclic, and NitrogenContaining Compounds on Oxidative Deamination of Polyamines in a Cell-Free Test System

Hilal, A, Goryachev, V.A, Blagonravov, M.L, Korzun, I.A, Sokuev, R.I, Syatkin, S.P, Sungrapova, K.Yu

2024

Overview

We studied the effects of some nitrogen-containing, heterocyclic, and cyclic compounds on the rate of oxidative deamination of polyamines and putrescine in tissues with a high proliferation rate. For this purpose, the specific activities of the main enzymes of polyamine oxidative degradation-spermine oxidase (SMO), polyamine oxidase (PAO), and diamine oxidase (DAO) were determined using a cell-free test system from regenerating rat liver. The compounds methyl 2-(5-formylfuran-2-yl)benzoate and 2,7-bis-[2-(diethylamino)ethoxy]- 9H-fluoren-9-one (and in the form of dihydrochloride) showed mainly activating effect on oxidative degradation of putrescine, spermidine, and spermine, which indirectly indicates their antiproliferative effect. Nitrogen-free compounds inhibited this process, thus exhibiting potentially carcinogenic properties. Correlations were calculated for activity of DAO, PAO, and SMO with 5 topological indices: Wiener (W), Rouvray (R), Balaban (J) in the Trinaistich modification, detour (Ip), and electropy (Ie). The highest dependence was noted for DAO and the Balaban index (R=-0.55), for PAO and the detour index (R=0.78), and for SMO and the electropy index (R=0.53). The remaining dependencies showed insignificant correlation strength. Key Words: polyamines; diamine oxidase; polyamine oxidase; spermine oxidase; heterocyclic compounds

• Publisher: Springer
• Subject: Heterocyclic compounds / Oxidases / Polyamines / Testing equipment