Journal Article

The m.sup.5C reader Ybx1 regulates embryonic cortical neurogenesis by promoting progenitor cell cycle progression

Zhang, Yanbo, Yang, Zhuoxuan, Xu, Jiuzhou, Zhang, Jian, Shui, Yuxuan, Lu, Xibin, Ji, Sheng-Jian, Che, Pengfei, She, Yuanchu, Yu, Jun, Zhang, Pingrui

2025

Overview

The reversible epitranscriptomic mark, 5-methylcytosine (m.sup.5 C) modification, is implicated in numerous cellular processes, but its role in neural development remains largely unexplored. In this study, we discovered high expression of the m.sup.5 C reader Ybx1 in the developing mouse cortex. To elucidate its role in cortical development, Ybx1 was ablated in embryonic cortical neural stem cells (NSCs). Interestingly, conditional knockout (cKO) of Ybx1 led to perinatal mortality in mice, along with abnormal cortical development. Cortical progenitor cells lacking Ybx1 exhibited impaired proliferation and differentiation. Multi-omics analysis identified the target mRNAs of Ybx1, which encode the key cell cycle regulatory proteins converging on cyclin D2 (Ccnd2). Ybx1 was found to regulate the stability of its target transcripts. Both knockdown and overexpression of Ybx1 targets via in utero electroporation confirmed that they mediated Ybx1 regulation of proliferation and differentiation of neural precursor cells. Further analysis showed that the G1 to S phase transition in cortical progenitor cells is delayed in the Ybx1 cKO. This study highlights the crucial function of the m.sup.5 C reader protein Ybx1 in promoting cell cycle progression of the embryonic cortical progenitors, essential for proper cortical development.

• Publisher: Public Library of Science
• Subject: Analysis / Binding proteins / Brain / Brain research / Cell cycle / Cerebral cortex / Embryonic development / Gene expression / Genetic aspects / Neurogenesis / Physiological aspects