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BoagammaPLI: Structural and functional characterization of the gamma phospholipase A2 plasma inhibitor from the non-venomous Brazilian snake Boa constrictor
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BoagammaPLI: Structural and functional characterization of the gamma phospholipase A2 plasma inhibitor from the non-venomous Brazilian snake Boa constrictor
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Journal Article
BoagammaPLI: Structural and functional characterization of the gamma phospholipase A2 plasma inhibitor from the non-venomous Brazilian snake Boa constrictor
2020
Overview
Plasma in several organisms has components that promote resistance to envenomation by inhibiting specific proteins from snake venoms, such as phospholipases A.sub.2 (PLA.sub.2 s). The major hypothesis for inhibitor's presence would be the protection against self-envenomation in venomous snakes, but the occurrence of inhibitors in non-venomous snakes and other animals has opened new perspectives for this molecule. Thus, this study showed for the first time the structural and functional characterization of the PLA.sub.2 inhibitor from the Boa constrictor serum (Boa[gamma]PLI), a non-venomous snake that dwells extensively the Brazilian territory. Therefore, the inhibitor was isolated from B. constrictor serum, with 0.63% of recovery. SDS-PAGE showed a band at ~25 kDa under reducing conditions and ~20 kDa under non-reducing conditions. Chromatographic analyses showed the presence of oligomers formed by Boa[gamma]PLI. Primary structure of Boa[gamma]PLI suggested an estimated molecular mass of 22 kDa. When Boa[gamma]PLI was incubated with Asp-49 and Lys-49 PLA.sub.2 there was no severe change in its dichroism spectrum, suggesting a non-covalent interaction. The enzymatic assay showed a dose-dependent inhibition, up to 48.2%, when Boa[gamma]PLI was incubated with Asp-49 PLA.sub.2, since Lys-49 PLA.sub.2 has a lack of enzymatic activity. The edematogenic and myotoxic effects of PLA.sub.2 s were also inhibited by Boa[gamma]PLI. In summary, the present work provides new insights into inhibitors from non-venomous snakes, which possess PLIs in their plasma, although the contact with venom is unlikely.
Publisher
Public Library of Science
Subject
