Journal Article

Antibacterial Spirotetronate Polyketides from an IActinomadura/I sp. Strain A30804

Leong, Chung-Yan, Yang, Lay-Kien, Ng, Veronica W, Tan, Zann Y, Chin, Elaine J, Kanagasundaram, Yoganathan, Ng, Siew-Bee, Seow, Deborah C, Wibowo, Mario, Ching, Kuan-Chieh

2022

Overview

Large scale cultivation and chemical investigation of an extract obtained from Actimonadura sp. resulted in the identification of six previously undescribed spirotetronates (pyrrolosporin B and decatromicins C–G; 7–12), along with six known congeners, namely decatromicins A–B (1–2), BE-45722B–D (3–5), and pyrrolosporin A (6). The chemical structures of compounds 1–12 were characterized via comparison with previously reported data and analysis of 1D/2D NMR and MS data. The structures of all new compounds were highly related to the spirotetronate type compounds, decatromicin and pyrrolosporin, with variations in the substituents on the pyrrole and aglycone moieties. All compounds were evaluated for antibacterial activity against the Gram-negative bacteria, Acinetobacter baumannii and Gram-positive bacteria, Staphylococcus aureus and were investigated for their cytotoxicity against the human cancer cell line A549. Of these, decatromicin B (2), BE-45722B (3), and pyrrolosporin B (7) exhibited potent antibacterial activities against both Gram-positive (MIC[sub.90] between 1–3 μM) and Gram-negative bacteria (MIC[sub.90] values ranging from 12–36 μM) with weak or no cytotoxic activity against A549 cells.

• Publisher: MDPI AG
• Subject: Antibacterial agents