Asset Details
Do you wish to reserve the book?
5-HTsub.2A Receptor Knockout Mice Show Sex-Dependent Differences following Acute Noribogaine Administration
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
5-HTsub.2A Receptor Knockout Mice Show Sex-Dependent Differences following Acute Noribogaine Administration
Do you wish to request the book?
5-HTsub.2A Receptor Knockout Mice Show Sex-Dependent Differences following Acute Noribogaine Administration
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
5-HTsub.2A Receptor Knockout Mice Show Sex-Dependent Differences following Acute Noribogaine Administration
2024
Overview
Noribogaine (noribo) is the primary metabolite from ibogaine, an atypical psychedelic alkaloid isolated from the root bark of the African shrub Tabernanthe iboga. The main objective of this study was to test the hypothesis that molecular, electrophysiological, and behavioral responses of noribo are mediated by the 5-HT[sub.2A] receptor (5-HT[sub.2A]R) in mice. In that regard, we used male and female, 5-HT[sub.2A]R knockout (KO) and wild type (WT) mice injected with a single noribo dose (10 or 40 mg/kg; i.p.). After 30 min., locomotor activity was recorded followed by mRNA measurements by qPCR (immediate early genes; IEG, glutamate receptors, and 5-HT[sub.2A]R levels) and electrophysiology recordings of layer V pyramidal neurons from the medial prefrontal cortex. Noribo 40 decreased locomotion in male, but not female WT. Sex and genotype differences were observed for IEG and glutamate receptor expression. Expression of 5-HT[sub.2A]R mRNA increased in the mPFC of WT mice following Noribo 10 (males) or Noribo 40 (females). Patch-clamp recordings showed that Noribo 40 reduced the NMDA-mediated postsynaptic current density in mPFC pyramidal neurons only in male WT mice, but no effects were found for either KO males or females. Our results highlight that noribo produces sexually dimorphic effects while the genetic removal of 5HT[sub.2A]R blunted noribo-mediated responses to NMDA synaptic transmission.
Publisher
MDPI AG
Subject
