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Isolation and Characterization of a Newly Discovered Phage, V-YDF132, for Lysing IVibrio/I Iharveyi/I
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Isolation and Characterization of a Newly Discovered Phage, V-YDF132, for Lysing IVibrio/I Iharveyi/I
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Isolation and Characterization of a Newly Discovered Phage, V-YDF132, for Lysing IVibrio/I Iharveyi/I
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Journal Article
Isolation and Characterization of a Newly Discovered Phage, V-YDF132, for Lysing IVibrio/I Iharveyi/I
2022
Overview
A newly discovered lytic bacteriophage, V-YDF132, which efficiently infects the pathogenic strain of Vibrio harveyi, was isolated from aquaculture water collected in Yangjiang, China. Electron microscopy studies revealed that V-YDF132 belonged to the Siphoviridae family, with an icosahedral head and a long noncontractile tail. The phage has a latent period of 25 min and a burst size of 298 pfu/infected bacterium. V-YDF132 was stable from 37 to 50 °C. It has a wide range of stability (pH 5–11) and can resist adverse external environments. In addition, in vitro the phage V-YDF132 has a strong lytic effect on the host. Genome sequencing results revealed that V-YDF132 has a DNA genome of 84,375 bp with a GC content of 46.97%. In total, 115 putative open reading frames (ORFs) were predicted in the phage V-YDF132 genome. Meanwhile, the phage genome does not contain any known bacterial virulence genes or antimicrobial resistance genes. Comparison of the genomic features of the phage V-YDF132 and phylogenetic analysis revealed that V-YDF132 is a newly discovered Vibrio phage. Multiple genome comparisons and comparative genomics showed that V-YDF132 is in the same genus as Vibrio phages vB_VpS_PG28 (MT735630.2) and VH2_2019 (MN794238.1). Overall, the results indicate that V-YDF132 is potentially applicable for biological control of vibriosis.
Publisher
MDPI AG
