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A paper-based competitive lateral flow immunoassay for multi beta-agonist residues by using a single monoclonal antibody labelled with red fluorescent nanoparticles
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A paper-based competitive lateral flow immunoassay for multi beta-agonist residues by using a single monoclonal antibody labelled with red fluorescent nanoparticles
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A paper-based competitive lateral flow immunoassay for multi beta-agonist residues by using a single monoclonal antibody labelled with red fluorescent nanoparticles
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Journal Article
A paper-based competitive lateral flow immunoassay for multi beta-agonist residues by using a single monoclonal antibody labelled with red fluorescent nanoparticles
2018
Overview
An ultrasensitive paper based lateral flow assay is described for rapid and simultaneous fluorometric detection of several [beta]-agonists including clenbuterol and its chemical analogues (mabuterol, brombuterol, cimaterol, cimbuterol, bromchlorbuterol and banbuterol). A nonspecific monoclonal antibody (mAb) against clenbuterol and its analogues was prepared and employed in a competitive immunoassay where mAb conjugated to fluorescent nanoparticles and free [beta]-agonists compete for the binding sites. This enables rapid screening for the 7 [beta]-agonists in a single run that takes about 8 min. Detection limits for the seven [beta]-agonists are <50 pg g.sup.-1 of pork. Recoveries ranged from 69.5% to 102.4%, and relative standard deviations were ±15%. The assay was applied to the analysis of both using spiked and unspiked pork for [beta]-agonists, and the results compare well to those obtained by HPLC-MS.
Publisher
Springer
Subject
