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Inter- and Intraspecies Transfer of a Clostridium difficile Conjugative Transposon Conferring Resistance to MLSB
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Inter- and Intraspecies Transfer of a Clostridium difficile Conjugative Transposon Conferring Resistance to MLSB
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Journal Article
Inter- and Intraspecies Transfer of a Clostridium difficile Conjugative Transposon Conferring Resistance to MLSB
2014
Overview
Resistance to the macrolide-lincosamide-streptogramin B group of antibiotics in
Clostridium difficile
is generally due to
erm
(B) genes. Tn
6194
, a conjugative transposon initially detected in PCR-ribotype 027 isolates, is an
erm
(B)-containing element also detected in other relevant
C. difficile
PCR-ribotypes. In this study, the genome of a
C. difficile
PCR-ribotype 001 strain was sequenced, and an element with two nucleotidic changes compared to Tn
6194
was detected. This element was transferred by filter mating assays to recipient strains of
C. difficile
belonging to PCR-ribotype 009 and 027 and to a recipient strain of
Enterococcus faecalis
. Transconjugants were characterized by Southern blotting and genome sequencing, and integration sites in all transconjugants were identified. The element integrated the genome of
C. difficile
at different sites and the genome of
E. faecalis
at a unique site. This study is the first molecular characterization of an
erm
(B)-containing conjugative transposon in
C. difficile
and provides additional evidence of the antibiotic resistance transmission risk among pathogenic bacteria occupying the same human intestinal niche.
Publisher
Mary Ann Liebert, Inc,SAGE Publications,Mary Ann Liebert
Subject
/ Anti-Bacterial Agents - pharmacology
/ Bacteria
/ Biochemistry, Molecular Biology
/ Clostridium difficile - drug effects
/ Clostridium difficile - genetics
/ DNA Transposable Elements - genetics
/ Drug Resistance, Microbial - genetics
/ Enterococcus faecalis - genetics
/ Genes
/ Genome, Bacterial - genetics
