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A differentially regulated AP2/ERF transcription factor gene cluster acts downstream of a MAP kinase cascade to modulate terpenoid indole alkaloid biosynthesis in Catharanthus roseus
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A differentially regulated AP2/ERF transcription factor gene cluster acts downstream of a MAP kinase cascade to modulate terpenoid indole alkaloid biosynthesis in Catharanthus roseus
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A differentially regulated AP2/ERF transcription factor gene cluster acts downstream of a MAP kinase cascade to modulate terpenoid indole alkaloid biosynthesis in Catharanthus roseus
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Journal Article
A differentially regulated AP2/ERF transcription factor gene cluster acts downstream of a MAP kinase cascade to modulate terpenoid indole alkaloid biosynthesis in Catharanthus roseus
2017
Overview
Catharanthus roseus produces bioactive terpenoid indole alkaloids (TIAs), including the chemotherapeutics, vincristine and vinblastine. Transcriptional regulation of TIA biosynthesis is not fully understood.
The jasmonic acid (JA)-responsive AP2/ERF transcription factor (TF), ORCA3, and its regulator, CrMYC2, play key roles in TIA biosynthesis. ORCA3 forms a physical cluster with two uncharacterized AP2/ERFs, ORCA4 and 5. Here, we report that (1) the ORCA gene cluster is differentially regulated; (2) ORCA4, while overlapping functionally with ORCA3, modulates an additional set of TIA genes. Unlike ORCA3, ORCA4 overexpression resulted in dramatic increase of TIA accumulation in C. roseus hairy roots. In addition, CrMYC2 is capable of activating ORCA3 and co-regulating TIA pathway genes concomitantly with ORCA3.
The ORCA gene cluster and CrMYC2 act downstream of a MAP kinase cascade that includes a previously uncharacterized MAP kinase kinase, CrMAPKK1. Overexpression of CrMAPKK1 in C. roseus hairy roots upregulated TIA pathways genes and increased TIA accumulation.
This work provides detailed characterization of a TF gene cluster and advances our understanding of the transcriptional and post-translational regulatory mechanisms that govern TIA biosynthesis in C. roseus.
Publisher
New Phytologist Trust,Wiley Subscription Services, Inc
Subject
/ Catharanthus roseus (Madagascar periwinkle)
/ Cyclopentanes - pharmacology
/ Gene Expression Regulation, Plant - drug effects
/ MAP Kinase Signaling System - drug effects
/ MAP Kinase Signaling System - genetics
/ mitogen-activated protein kinase
/ mitogen-activated protein kinase kinase
/ Phosphorylation - drug effects
/ Protein Binding - drug effects
/ Protein Transport - drug effects
/ Roots
/ Secologanin Tryptamine Alkaloids - metabolism
/ Structure-Activity Relationship
/ terpenoid indole alkaloids (TIAs)
/ Transcription Factors - chemistry
/ Transcription Factors - genetics
/ Transcription Factors - metabolism
/ Transcriptional Activation - drug effects
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