Asset Details

MbrlCatalogueTitleDetail

Do you wish to reserve the book?

Phage Display Derived Antibodies Against Antimicrobial Peptide FsPDF2 Reveal Stress Response in European Beech

by Schnitzler, Jörg‐Peter , Schubert, Maren , Schumacher, Jörg , Hust, Michael , Hu, Bin , Heine, Philip Alexander , Hanke‐Uhe, Manuel , Engelmann, Susanne , Meier, Doris , Perl, Asta , Dübel, Stefan , Shahid, Afsheen , Hänsch, Robert , Linke, Tanja , Lehmler, Nina , Kucklick, Martin , Polten, Saskia , Winkler, Jana Barbro , Schäckermann, Dorina , Korn, Janin , Becker, Marlies , Rennenberg, Heinz , Kaufholdt, David , Priess, Valeska , Oliphant, Kevin Dennis , Kistner, Sarah , Goerke, Nicole , Nosenko, Tetyana

in Abiotic stress / Amino acids / AMP quantification / Annotations / Antibodies / Antibodies, Monoclonal - immunology / Antigens / Antimicrobial peptides / Antimicrobial Peptides - immunology / Antimicrobial Peptides - metabolism / Beech / Cell cycle / Climate change / crude plant extract ELISA / Defensins / Defensins - immunology / Defensins - metabolism / Drought / drought/biotic stress / Enzyme-Linked Immunosorbent Assay / Fagus - immunology / Fagus - metabolism / Fagus - physiology / forest stress diagnostic / Genes / Genomes / Genomics / Herbivores / Immune system / Monoclonal antibodies / Pathogens / Peptides / Phage display / phage display recombinant antibodies / Phages / plant defensins / Plant extracts / Plant immunity / Plant Proteins - immunology / Plant Proteins - metabolism / Plant tissues / Protein structure / Proteins / Stress response / Stress, Physiological / Tertiary structure

2026

Yes Please

Hey, we have placed the reservation for you!

By the way, why not check out events that you can attend while you pick your title.

Oops! Something went wrong.

Looks like we were not able to place the reservation. Kindly try again later.

Are you sure you want to remove the book from the shelf?

Phage Display Derived Antibodies Against Antimicrobial Peptide FsPDF2 Reveal Stress Response in European Beech

2026

Confirm

Do you wish to request the book?

Phage Display Derived Antibodies Against Antimicrobial Peptide FsPDF2 Reveal Stress Response in European Beech

2026

Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy

How would you like to get it?

Submit

We have requested the book for you!

Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.

Oops! Something went wrong.

Looks like we were not able to place your request. Kindly try again later.

Journal Article

Phage Display Derived Antibodies Against Antimicrobial Peptide FsPDF2 Reveal Stress Response in European Beech

Schnitzler, Jörg‐Peter,

Schubert, Maren,

Schumacher, Jörg,

Hust, Michael,

Hu, Bin,

Heine, Philip Alexander,

Hanke‐Uhe, Manuel,

Engelmann, Susanne,

Meier, Doris,

Perl, Asta,

Dübel, Stefan,

Shahid, Afsheen,

Hänsch, Robert,

Linke, Tanja,

Lehmler, Nina,

Kucklick, Martin,

Polten, Saskia,

Winkler, Jana Barbro,

Schäckermann, Dorina,

Korn, Janin,

Becker, Marlies,

Rennenberg, Heinz,

Kaufholdt, David,

Priess, Valeska,

Oliphant, Kevin Dennis,

Kistner, Sarah,

Goerke, Nicole,

Nosenko, Tetyana

2026

Overview

Plant defensins (PDFs) are cysteine‐rich antimicrobial peptides (AMPs) that are important components of plant immunity. They occur constitutively in various plant tissues but are also upregulated upon stress. Therefore, these molecules are of great interest as markers for the diagnosis of early forest stress response in plants at the molecular level. PDFs are small peptides ( 5 kDa) with a compact tertiary structure, requiring specific protocols and dedicated antibodies for detection by quantitative ELISA. We developed monoclonal recombinant antibodies using phage display in solution against the correctly folded antigen defensin FsPDF2 from beech (Fagus sylvatica) and analysed the antibody–antigen interaction in silico with AlphaFold 3. In a proof‐of‐principle study, we investigated the FsPDF2 stress response to abiotic (drought) and biotic (gall midge) stresses. Notably, we established an assay for defensin quantification in crude plant extract, detecting for the first time natively folded proteins in a specific sandwich ELISA. Our antibody generation strategy can be transferred by practitioners to other small antimicrobial peptides (AMP), paving the way to study this group of proteins and their corresponding stress response comprehensively.

Share this book

Add to My Shelf

Publisher

John Wiley & Sons, Inc,Wiley

Subject

/ Antibodies, Monoclonal - immunology

/ Antigens