Journal Article

Assessment of the Antimicrobial and Antioxidant Activities and Total Phenolic Content of Crude Extracts from Senna Italic, Sudan

Fadlalla, Ahmed Ali Mustafa, Omer, Haifa A. A

2024

Overview

The objective of the present study was the assessment of the antimicrobial and antioxidant activities of Senna italic., from n-hexane, chloroform, ethyl acetate and methanol extracts. In addition, the total polyphenol, flavonoids and tannins contents of these extracts were determined and; were screened for their antimicrobial activity using cup diffusion assay against four standard strains of bacteria, two Gram-positive strains (Bacillus subtitles and Staphylococcus aurous), two Gram-negative bacterial strains (Escherichia coli and Pseudomonas aeruginosa) and two fungal strains (Aspergillums Niger and Candida barbicans). Antioxidant activity was screened using 2,2-Di(4-tert-octylphenyl)-1-picryl-hydrazyl stable free radical (DPPH). Total polyphenol, flavonoids and tannins contents were determined by spectrophotometric assays. Generally, the results of antimicrobial activity showed that extracts of the plant exhibited better antifungal activity than antibacterial. The highest antifungal activity against Angier and Candida albinos., was recorded from the methanol extract of Senna italic., (inhibition zone (IZ)= 22±1.4 and 20±1.1 mm respectively), flowed by ethyl acetate extract against C.albicans., (IZ= 14±0.2 mm); while the highest antibacterial activity of the methanol extract against Ecbolic., gave the best activity (IZ= 13±0.4 mm), flowed by methanol and ethyl acetate extracts against Pseudomonas aeruginosa., and Bacillus stabiles (IZ= 12±0.3 mm) from the disc diffusion method. The highest scavenging radical activity was obtained from the methanol extract and gave (24%). Quantitative analysis revealed the total polyphenol value, with the highest result shown in ethyl acetate (188.6 mg Gallic acid equivalent (GAE)/ g) and the flowed methanol extract (70.6±0.04 mg (GAE)/ g).The highest total flavonoids content value was recorded from the chloroform extract (356.66±0.43 mg quercetin equivalent/ g), flowed by methanol extract (269.33 mg quercetin equivalent/ g),while the highest total tannins content value was found in methanol extract (38±0.03 mg tannic acid equivalents/ g).

• Publisher: جامعة أم درمان الإسلامية - معهد البحوث والدراسات الإستراتيجية
• Subject: البكتيريا والفطريات / مضادات الأكسدة / مضادات الميكروبات / نبات السنا