Paper

Retinal resuscitation in post-mortem eyes

Byrne, Eimear M., Cosma, Maria Pia, Balashov, Mark, Lee, Jack, Rodriguez, Daniel Caravaca, Angles, Marta Cadevall, Merino, Manuel Fernández, Rotkevich, Mikhail, Marin Figuera, Jose Alejandro, Farah, Nairouz, Solano, Miguel Guerra, Ibrahim, Mohammed Salim, Sandiumenge, Alberto, Marano, Ricardo Casaroli, Puig Galy, Javier José, Vicino, Umberto Di, Mandel, Yossi

2026

Overview

Vision loss compromises the quality of life of millions of people worldwide. Currently, vision-restoring therapies are lacking. Post-mortem preservation of human eyes may enable whole eye transplantation (WET) or therapeutic development. We developed an approach to cannulate the ophthalmic artery and perfuse post-mortem pig and human eyes using a custom-built device, Eye-in-Care-Box (ECaBox). Retinal vasculature imaging and reconstruction were used to assess perfusion efficacy using a supervised deep learning model. Without intervention, the retina degrades post-mortem, whereas perfusion preserves retinal structure and cell viability for up to 24-hours. Eyes were perfused within 30 minutes post-extraction, and regained light-responses persisted for up to 10 hours after death, challenging the notion that response to light ends at death. Our findings show that intact eyes can be resuscitated and preserved outside the body, supporting the ECaBox platform for WET preservation and pre-clinical therapeutic testing. Ultimately, ECaBox may bring vision-restoring treatments to patients.

• Publisher: Cold Spring Harbor Laboratory
• Subject: Neuroscience