Journal Article

Mechanisms of antiprostate cancer by gum mastic： NF-kB signal as target

Mei-lan HE Ang LI Chun-su XU Shun-li WANG Meng-jie ZHANG Hua GU Yao-qin YANG Hui-hong TAO

2007

Overview

Aim： To study the effect of gum mastic, a natural resin, on the proliferation of androgen-independent prostate cancer PC-3 cells, and further investigate the mechanisms involved in this regulatory system, taking nuclear factor rd3 （NF-kB） signal as the target. Methods： 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide （MTT） assay and a flow cytometer were used to detect the effect of gum mastic on the proliferation of PC-3 cells. Then, reporter gene assay, RT-PCR, and Western blotting were carried out to study the effects of gum mastic on the NF-kB protein level and the NF-kB signal pathway. The expression of genes involved in the NF-kB signal pathway, including cyclin Dl, inhibitors of kBs （Ik Bα）, and phosphorylated Akt （p-AKT）, were measured. In addition, transient transfection assays with the 5x NF-kB consensus sequence promoter was also used to test the effects of gum mastic. Results： Gum mastic inhibited PC-3 cell growth and blocked the PC-3 cell cycle in the Gl phase. Gum mastic also suppressed NF-kB activity in the PC-3 cells. The expression of cyclin Dl, a crucial cell cycle regulator and an NF-kB downstream target gene, was reduced as well. Moreover, gum mastic decreased the p-AKT protein level and increased the InBα protein level. Conclusion： Gum mastic inhibited the proliferation and blocked the cell cycle progression in PC-3 cells by suppressing NF-kB activity and the NF-kB signal pathway.

• Subject: NF-kB信号 / 乳香树脂 / 前列腺癌 / 抗癌机理