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尾悬吊模拟失重大鼠卵巢组织中缺氧诱导因子-1α表达变化
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Journal Article
尾悬吊模拟失重大鼠卵巢组织中缺氧诱导因子-1α表达变化
2012
Overview
目的研究模拟失重环境下大鼠卵巢组织中缺氧诱导因子-1α（HIF-1α）的表达变化。方法取性成熟期健康雌性Wistar大鼠60只作为研究对象,体重200±20g,随机分为10组（n=6）,按模拟失重时相分为悬吊0.25、0.5、1、2、3、5、7、14、21d组和0d组（对照组）。采用尾悬吊法建立模拟失重动物模型。各组大鼠卵巢组织中HIF-1α的表达分别应用免疫组织化学和RT-PCR方法进行检测。结果各实验组及对照组大鼠卵巢卵泡卵母细胞、颗粒细胞和膜细胞中均可见HIF-1α阳性染色细胞。对照组大鼠卵巢HIF-1α染色阳性颗粒主要位于细胞质中,悬吊0.25d和0.5d组大鼠卵巢卵母细胞、颗粒细胞和膜细胞的HIF-1α颗粒由细胞质向细胞核转移;尾悬吊1d组HIF-1α表达情况与对照组接近,HIF-1α核染色消失。尾悬吊0.25d、0.5d大鼠卵巢HIF-1αmRNA表达水平显著高于对照组（P〈0.05）,持续尾悬吊1d以后各组卵巢HIF-1α的蛋白和mRNA水平逐渐恢复至对照组水平。结论尾悬吊模拟失重使大鼠卵巢组织中HIF-1α蛋白及mRNA表达发生明显变化,早期同步过表达,提示卵巢HIF-1α表达变化与失重应激反应和失重耐受有密切关系。
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