Journal Article

Jiaweisinisan facilitates neurogenesis in the ippocampus after stress damage

Lili Wu Chuanlian Ran Shukao Liu Lizhen Liao Yanling Chen Hualei Guo Weikang Wu Can Yan

2013

Overview

The traditional Chinese medicine Jiaweisinisan has antidepressant effects, and can inhibit hypothalamus-pituitary-adrenal gland axis hyperactivity in stress-induced depression. In this study, rat hippocampal neural precursor cells were cultured in serum-free medium in vitro and a stress damage model was established with 120 IJM corticosterone. Cells were treated with 10% （v/v） Jiaweisinisan drug-containing serum and the corticosterone antagonist RU38486. Results of the 3-（4,5-dimethylthiazol-2-yl）-3,5-di-phenytetrazoliumromide assay showed that both Jiaweisinisan drug-containing serum and RU38486 promoted the proliferation of neural precursor cells after corticosterone exposure. Immunofluorescence detection showed that after Jiaweisinisan drug-containing serum and RU38486 treatment, the 5-bromo-2-deoxyuridine/terminal deoxynucleotidyl transferase dUTP nick end labeling ratio in hippocampal neural precursor cells significantly increased, and the apoptotic rates of glial cells reduced, and neuron-like cell differentiation from neural precursor cells significantly increased. Our experimental findings indicate that Jiaweisinisan promotes hippocampal neurogenesis after stress damage.

• Subject: 二甲基吡啶 / 免疫荧光检测 / 加味 / 应激损伤 / 无血清培养基 / 生后 / 神经前体细胞 / 脱氧核苷酸